Mimix™ ALK RET ROS RNA fusion, FFPE Reference Standard

The ALK-RET-ROS1 targeted FFPE RNA Fusion Reference Standards are a highly-characterized, biologically-relevant quality control material used to assess the performance of NGS, RT-PCR and RT-qPCR assays aimed at detecting gene fusions. Each section contains formalin-fixed, paraffin-embedded (FFPE) cell line multiplex verified to contain EML4-ALK, CCDC6-RET and SLC34A2-ROS1 fusions.

Targeted NGS, endpoint RT-PCR and RT-qPCR platforms give researchers much greater power when profiling tumor samples. However, they present challenges surrounding sample variability, RNA extraction procedures, RNA quantification, platform bias, and potential failure to detect biomarkers. To meet this need, Horizon has developed a targeted EML4-ALK, CCDC6-RET and SLC34A2-ROS1 verified cell line multiplex in an FFPE format. This renewable and highly-characterized biologically-relevant reference materials contain cell lines that were either engineered or clonally derived from a fusion background. The utilization of clonal cell populations derived from single cell dilution and controlled manufacturing environment ensure reproducible amplifiable levels of EML4-ALKv1, CCDC6-RET and SLC34A2-ROS1 allowing for a consistent external quality control.

Achieve end-to-end process validation with a highly homogeneous cell suspension that yields a consistent and reproducible quantity of RNA.With this product you are able to:

  • Evaluate your workflow integrity from pre-analytical RNA extraction through to fusion detection
  • Optimize and validate new targeted RNA panels and routinely monitor the performance of your assay
  • Assess the performance of your RNA-seq, endpoint RT-PCR or RT-qPCR aimed at detecting EML4-ALK, CCDC6-RET and SLC34A2-ROS1 fusions
  • Determine analytical variability and reproducibility between platforms, laboratories, operators and assays
  • Ensure confidence in your clinical workflow, from RNA extraction to analysis

Breakpoint sequence data

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Technical Data

Format: FFPE

Genes Covered: EML4-ALK, CCDC6-RET and SLC34A2-ROS1

Fixation Method: 10% Formalin

Section Size: 10µm

 

Product Information

Unit Size: 1 FFPE Section

 

General Information

Storage: 4°C

Expiry: See all product shelf life information

 

Quality Control

Extractable RNA / Expected Yield: ≥ 100 ng RNA per section using the Maxwell RSC RNA FFPE Purification Kit from Promega

Biomarker-specific mRNA amplifiability: Presence of EML4-ALK, CCDC6-RET and SLC34A2-ROS1 RNA fusion transcripts confirmed by endpoint RT-PCR

TATA-binding protein (TBP) mRNA amplifiability control: RNA expression confirmed by endpoint RT-PCR

Quantification: Qubit RNA HS kit from ThermoFisher as per manufacturer’s instructions

Intended use: For assay developers and molecular diagnostic labs for routine performance monitoring of molecular biology assays. Research use only. Not for diagnostic procedures.