Horizon FFPE Reference standards are cell-line derived making them reproducible, renewable, and affordable experimental controls for molecular diagnostic assays. They are formulated to mimic patient samples and serve as excellent quality controls by acting as performance indicators for the whole diagnostic workflow.

Technical Information

Horizon FFPE standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. Using a proprietary method of mild fixation and homogenous paraffin embedding, we generate highly reproducible and consistent FFPE curls. Our standards yield high molecular weight DNA which can be analyzed in a wide-range of assays including qPCR, Sanger sequencing and next-generation sequencing.

Product Information

Product Description: BRAF V600K Reference Standard, 50%

Catalog number: HD268

Gene: BRAF

Amino Acid Change: V600K

Nucleotide change: c.1798_1799GT>AA

Variant Allele Frequency: 50%

Cosmic Genomic Mutation ID: COSV56057713

Cosmic Legacy ID: COSM473

GRCh38 position: 7:140753336-140753337

Format: FFPE

Unit Size: 15 µm section

Fixation Method: 4% PFA (w/v)

Expected DNA Yield: ≥ 400ng Using Maxwell RSC Plus FFPE DNA Extraction kit

Storage: 4°C

Shipping: Ambient

Expiry: See all product shelf-life information

Cell Line Background: HCT116

Exome Sequencing Data: Download additional sequencing data from the parental cell

Quality Control

Genotype and Allelic Frequency: Droplet Digital PCRTM

DNA integrity: Agarose gel electrophoresis

Quantification: Quantifluor™

Manufacturing Quality: ISO 13485:2016

Use: Research Use Only

Cell Line Characterization

Chromatogram:

Chromatograph showing heterozygosity for the B-Raf V600K mutation within B-Raf exon 15 (COSMIC mutation number: 473)

Primers: