Horizon FFPE Reference standards are cell-line derived making them reproducible, renewable, and affordable experimental controls for molecular diagnostic assays. They are formulated to mimic patient samples and serve as excellent quality controls by acting as performance indicators for the whole diagnostic workflow.
Horizon FFPE standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. Using a proprietary method of mild fixation and homogenous paraffin embedding, we generate highly reproducible and consistent FFPE curls. Our standards yield high molecular weight DNA which can be analyzed in a wide-range of assays including qPCR, Sanger sequencing and next-generation sequencing.
Product Description: BRAF V600K Reference Standard, 50%
Catalog number: HD268
Amino Acid Change: V600K
Nucleotide change: c.1798_1799GT>AA
Variant Allele Frequency: 50%
Cosmic Genomic Mutation ID: COSV56057713
Cosmic Legacy ID: COSM473
GRCh38 position: 7:140753336-140753337
Unit Size: 15 µm section
Fixation Method: 4% PFA (w/v)
Expected DNA Yield: ≥ 400ng Using Maxwell RSC Plus FFPE DNA Extraction kit
Cell Line Background: HCT116
Exome Sequencing Data: Download additional sequencing data from the parental cell
Genotype and Allelic Frequency: Droplet Digital PCRTM
DNA integrity: Agarose gel electrophoresis
Manufacturing Quality: ISO 13485:2016
Use: Research Use Only
Cell Line Characterization
Chromatograph showing heterozygosity for the B-Raf V600K mutation within B-Raf exon 15 (COSMIC mutation number: 473)
We accept orders using the following methods.
Our online catalog and ordering system can be used to search our full menu of available Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.
If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order. We recommend that you use our online system to identify the products you require and their respective “HD” product codes.
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- Product code(s) of the item(s) you wish to order (e.g. "HD123")
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Shipping information for all territories can be found on our Shipping Charges page. This includes Shipping and Handling fees, as well as shipping conditions for each product range.
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