Mimix™ BRAF V600K 50%, FFPE Reference Standard

The BRAF V600K Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of assays that detect somatic mutations, such as Sanger and qPCR sequencing assays.

DISCONTINUATION NOTE: HD268 will be discontinued by 03-Apr-2025. Last order request date is on/before 24-Mar-2025. We are working to include this mutation in an upcoming BRAF multiplex panel which will be available from May/June 2025. In the meantime, you can use the following alternate products HD123/HD274. For any further queries please contact us. If you face any issues while placing the order, please reach out to orders@horizondiscovery.com or your local sales team or distributor.

Horizon FFPE Reference standards are cell-line derived making them reproducible, renewable, and affordable experimental controls for molecular diagnostic assays. They are formulated to mimic patient samples and serve as excellent quality controls by acting as performance indicators for the whole diagnostic workflow.

 

Technical Information

Horizon FFPE standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. Using a proprietary method of mild fixation and homogenous paraffin embedding, we generate highly reproducible and consistent FFPE curls. Our standards yield high molecular weight DNA which can be analyzed in a wide-range of assays including qPCR, Sanger sequencing and next-generation sequencing.

 

Product Information

Product Description: BRAF V600K Reference Standard, 50%

Catalog number: HD268

Gene: BRAF

Amino Acid Change: V600K

Nucleotide change: c.1798_1799GT>AA

Variant Allele Frequency: 50%

GRCh38 position: 7:140753336-140753337

Format: FFPE

Unit Size: 15 µm section

Fixation Method: 4% PFA (w/v)

Expected DNA Yield: ≥ 400ng Using Maxwell RSC Plus FFPE DNA Extraction kit

Storage: 4°C

Shipping: Ambient

Expiry: See all product shelf-life information

 

Quality Control

Genotype and Allelic Frequency: Droplet Digital PCRTM

DNA integrity: Agarose gel electrophoresis

Quantification: Quantifluor™

Manufacturing Quality: ISO 13485:2016

Intended use: For assay developers and molecular diagnostic labs for routine performance monitoring of molecular biology assays. Research use only. Not for diagnostic procedures.