KRAS G12D Reference Standard, 5%

The KRAS G12D Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of assays that detect somatic mutations, such as Sanger and qPCR sequencing assays.

NOTE: HD119 will be discontinued by 20-Aug-2024. Last order request date is on/before 05-Aug-2024. This product will be replaced with HD301. For further information or questions, please contact us.

Horizon FFPE Reference standards are cell-line derived making them reproducible, renewable, and affordable experimental controls for molecular diagnostic assays. They are formulated to mimic patient samples and serve as excellent quality controls by acting as performance indicators for the whole diagnostic workflow.

Technical Information

Horizon FFPE standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. Using a proprietary method of mild fixation and homogenous paraffin embedding, we generate highly reproducible and consistent FFPE curls. Our standards yield high molecular weight DNA which can be analyzed in a wide-range of assays including qPCR, Sanger sequencing and next-generation sequencing.

Product Information

Product Description: KRAS G12D Reference Standard, 5%

Catalog number: HD119

Gene: KRAS

Amino Acid Change: G12D

Nucleotide change: c.34G>A

Variant Allele Frequency: 5%

GRCh38 position: 12:25245350

Format: FFPE

Unit Size: 15 µm section

Fixation Method: 4% PFA (w/v)

Expected DNA Yield: ≥ 400ng Using Maxwell RSC Plus FFPE DNA Extraction kit

Storage: 4°C

Shipping: Ambient

Expiry: See all product shelf-life information

Exome Sequencing Data: Download additional sequencing data from the parental cell

Quality Control

Genotype and Allelic Frequency: Droplet Digital PCRTM

DNA integrity: Agarose gel electrophoresis

Quantification: Quantifluor™

Manufacturing Quality: ISO 13485:2016

Use: Research Use Only