Multiplex I cfDNA in Synthetic Matrix II
The most flexible reference standard for cfDNA recovery
Optimal synthetic matrix enables extraction recovery of cfDNA by multiple purification methods. Clinically-relevant mutations in an artificial matrix give you full control of your cfDNA workflow, reducing the likelihood of a false negative or positive due to incorrect assay loading. Multiplex I cfDNA Reference Standard Set in Synthetic Matrix contains 8 onco-relevant mutations to measure your extraction efficiency.
The Multiplex I cfDNA Reference Standard Set in artificial matrix covers multiple engineered single nucleotide variants (SNVs/SNPs) with 8 mutations at 5%, 1%, and 0.1% allelic frequencies.
For information on specific gene copies detected in each product batch, download the product insert from the resources tab.
Technical data
Format: cfDNA
Genes covered: EGFR, KRAS, NRAS, PIK3CA
Cosmics: 1,500+
Allelic frequencies: 5%, 1%, 0.1% and 0% (100% wild type)
Buffer: Synthetic matrix II
Expected DNA yield: ≥120 ng DNA per mL of product using the QIAamp® Circulating Nucleic Acid Kit with 2 mL extraction input
Product information
Fragment size: 170bp
Unit size: 400ng per 1ml (4 vials total)
Extraction platform compatibility
| Product | Extraction platform | ||||
| Column based | Column/Bead | Bead Based | |||
| QIAamp Circulating Nucleic Acid Kit |
Macherey Nagel NucleoSnap Plasma kit |
QIAgen MinElute cfDNA kit |
MagMAX cfDNA isolation kit |
Maxwell RSC cfDNA isolation kit |
|
| Synthetic Matrix II | ✓ | ✓ |
✓ |
✓ |
✓ |
Verified mutations
| HD917 component expected allelic frequencies (%) | ||||||||
| Gene | Variant (AA) | CDS mutation | GRCh38 co-ordinates* | Cosmic ID | HD912 5% | HD913 1% | HD914 0.1% | HD915 100% WT |
| EGFR | T790M | c.2369C>T | 7:55181378 | COSV51765492 | 5 |
1 |
0.1 | 0 |
| EGFR | delE746-A750 | c.2235_2249del | 7:55174772-55174786 | COSV51765119 | 5 | 1 | 0.1 | 0 |
| EGFR | L858R | c.2573T>G | 7:55191822 | COSV51765161 | 5 | 1 | 0.1 | 0 |
| EGFR | V769-D770insASV/A767_V769dup | c.2300_2308dup | 7:55181317-55181318 | COSV51766549 | 5 | 1 | 0.1 | 0 |
| NRAS | Q61K | c.181C>A | 1:114713909 | COSV54736310 | 6.3 | 1.3 | 0.13 | 0 |
| NRAS | A59T | c.175G>A | 1:114713915 | COSV54743124 | 6.3 | 1.3 | 0.13 | 0 |
| PIK3CA | E545K | c.1633G>A | 3:179218303 | COSV55873239 | 6.3 | 1.3 | 0.13 | 0 |
| KRAS | G12D | c.35G>A | 12:25245350 | COSV55497369 | 6.3 | 1.3 | 0.13 | 0 |
*GRCh37 variant co-ordinates are shown in the Exome sequencing link below
Presence confirmed in parental cell line: EGFR G719S (20% allelic frequency)
Download additional information on variants present in the parental cell lines: Exome sequencing data
General information
Shipping conditions: Ambient (shipped with gel packs to ensure item maintains room temperature storage during transport)
Storage: 4°C
Expiration: See all product shelf life information
Quality control
Fragmentation size: D1000 DNA ScreenTape assay
Allelic frequency: Droplet Digital™ PCR
Quantification: Qubit dsDNA BR Assay (post-fragmentation)
