Multiplex I cfDNA in Synthetic Matrix II
The most flexible reference standard for cfDNA recovery
Optimal synthetic matrix enables extraction recovery of cfDNA by multiple purification methods. Clinically-relevant mutations in an artificial matrix give you full control of your cfDNA workflow, reducing the likelihood of a false negative or positive due to incorrect assay loading. Multiplex I cfDNA Reference Standard Set in Synthetic Matrix contains 8 onco-relevant mutations to measure your extraction efficiency.
The Multiplex I cfDNA Reference Standard Set in artificial matrix covers multiple engineered single nucleotide variants (SNVs/SNPs) with 8 mutations at 5%, 1%, and 0.1% allelic frequencies.
For information on specific gene copies detected in each product batch, download the product insert from the resources tab.
Technical data
Format: cfDNA
Genes covered: EGFR, KRAS, NRAS, PIK3CA
Cosmics: 1,500+
Allelic frequencies: 5%, 1%, 0.1% and 0% (100% wild type)
Buffer: Synthetic matrix II
Expected DNA yield: ≥120 ng DNA per mL of product using the QIAamp® Circulating Nucleic Acid Kit with 2 mL extraction input
Product information
Fragment size: 170bp
Unit size: 400ng per 1ml (4 vials total)
Extraction platform compatibility
Product | Extraction platform | ||||
Column based | Column/Bead | Bead Based | |||
QIAamp Circulating Nucleic Acid Kit |
Macherey Nagel NucleoSnap Plasma kit |
QIAgen MinElute cfDNA kit |
MagMAX cfDNA isolation kit |
Maxwell RSC cfDNA isolation kit |
|
Synthetic Matrix II | ✓ | ✓ |
✓ |
✓ |
✓ |
Verified mutations
HD917 component expected allelic frequencies (%) | ||||||||
Gene | Variant (AA) | CDS mutation | GRCh38 co-ordinates* | Cosmic ID | HD912 5% | HD913 1% | HD914 0.1% | HD915 100% WT |
EGFR | T790M | c.2369C>T | 7:55181378 | COSV51765492 | 5 |
1 |
0.1 | 0 |
EGFR | delE746-A750 | c.2235_2249del | 7:55174772-55174786 | COSV51765119 | 5 | 1 | 0.1 | 0 |
EGFR | L858R | c.2573T>G | 7:55191822 | COSV51765161 | 5 | 1 | 0.1 | 0 |
EGFR | V769-D770insASV/A767_V769dup | c.2300_2308dup | 7:55181317-55181318 | COSV51766549 | 5 | 1 | 0.1 | 0 |
NRAS | Q61K | c.181C>A | 1:114713909 | COSV54736310 | 6.3 | 1.3 | 0.13 | 0 |
NRAS | A59T | c.175G>A | 1:114713915 | COSV54743124 | 6.3 | 1.3 | 0.13 | 0 |
PIK3CA | E545K | c.1633G>A | 3:179218303 | COSV55873239 | 6.3 | 1.3 | 0.13 | 0 |
KRAS | G12D | c.35G>A | 12:25245350 | COSV55497369 | 6.3 | 1.3 | 0.13 | 0 |
*GRCh37 variant co-ordinates are shown in the Exome sequencing link below
Presence confirmed in parental cell line: EGFR G719S (20% allelic frequency)
Download additional information on variants present in the parental cell lines: Exome sequencing data
General information
Shipping conditions: Ambient (shipped with gel packs to ensure item maintains room temperature storage during transport)
Storage: 4°C
Expiration: See all product shelf life information
Quality control
Fragmentation size: D1000 DNA ScreenTape assay
Allelic frequency: Droplet Digital™ PCR
Quantification: Qubit dsDNA BR Assay (post-fragmentation)
Please see our terms and conditions of ordering.
If you need any support, advice or additional documentation then don't hesitate to contact us here. We accept orders using the following methods:
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If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order. We recommend that you use our online system to identify the products you require and their respective “HD” product codes. To order offline we will require the following information:
- Product code(s) of the item(s) you wish to order (e.g. "HD123")
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- Telephone number
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Shipping Information
Shipping information for all territories can be found on our Shipping Charges page. This includes Shipping and Handling fees, as well as shipping conditions for each product range.
If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please contact us.
Supporting Data
Comparison of extraction efficiencies with five cfDNA extraction kits when spiking cfDNA into Matrix I (HD817) or Matrix II (HD917)
Results demonstrate that Matrix II (HD917) has a higher compatibility with the most common extraction kits available for cfDNA, including bead and column based methods.