The Multiplex I cfDNA Reference Standard Set in artificial matrix covers multiple engineered single nucleotide variants (SNVs/SNPs) with 8 mutations at 5%, 1%, and 0.1% allelic frequencies.
For information on specific gene copies detected in each product batch, download the product insert from the resources tab.
Genes covered: EGFR, KRAS, NRAS, PIK3CA
Allelic frequencies: 5%, 1%, 0.1% and 0% (100% wild type)
Buffer: Synthetic matrix II
Expected DNA yield: ≥120 ng DNA per mL of product using the QIAamp® Circulating Nucleic Acid Kit with 2 mL extraction input
Fragment size: 170bp
Unit size: 400ng per 1ml (4 vials total)
Extraction platform compatibility
|Column based||Column/Bead||Bead Based|
|QIAamp Circulating Nucleic Acid Kit||Macherey Nagel NucleoSnap Plasma kit||QIAgen MinElute cfDNA kit||MagMAX cfDNA isolation kit||Maxwell RSC cfDNA isolation kit|
|Synthetic Matrix II|
|HD917 component expected allelic frequencies (%)|
|Gene||Variant (AA)||CDS mutation||GRCh38 co-ordinates*||Cosmic ID||HD912 5%||HD913 1%||HD914 0.1%||HD915 100% WT|
*GRCh37 variant co-ordinates are shown in the Exome sequencing link below
Presence confirmed in parental cell line: EGFR G719S (20% allelic frequency)
Exome sequencing data: Download additional information on variants present in the parental cell lines
Shipping conditions: Ambient (shipped with gel packs to ensure item maintains room temperature storage during transport)
Expiration: See all product shelf life information
Fragmentation size: D1000 DNA ScreenTape assay
Allelic frequency: Droplet Digital™ PCR
Quantification: Qubit dsDNA BR Assay (post-fragmentation)
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Comparison of extraction efficiencies with five cfDNA extraction kits when spiking cfDNA into Matrix I (HD817) or Matrix II (HD917)
Results demonstrate that Matrix II (HD917) has a higher compatibility with the most common extraction kits available for cfDNA, including bead and column based methods.