- Molecular reference standards
- OncoSpan FFPE
OncoSpan FFPE
This large panel NGS control contains over 380 variants across more than 152 key cancer genes.
OncoSpan is a well-characterized, cell line-derived Reference Standard containing over 380 variants across 152 key cancer genes. This includes 238 variants with a COSMIC ID and more than 28 INDELs (>22 deletions and 6 insertions, ranging from 1-16 base pairs). Variants are present between 1-100% allelic frequency (AF), with 50 variants present at ≤ 20% AF for LOD determination of your assay. Every batch of OncoSpan DNA has 25 variants confirmed by ddPCR, in addition to being fully exome sequenced (Batches before 54952 were sequenced on Illumina platform at 500x coverage using Agilent SureSelect Human All Exon V6 kit. Batches from 54952 are sequenced at Revvity Omics using Agilent CREV3 and Illumina platform). This provides you with an accurate and reliable truth set for comparison to your assay’s performance. You will receive a password upon ordering to download the raw NGS data for your batch (bam, bed and vcf files). Alternatively, for your convenience we have also listed the 386 high confidence variants in an easy-to-access excel document that can be downloaded.
Technical Data
Format: FFPE
Genes Covered: ABL1, AKT1, AKT2, ALK, APC, AR, ARID1A, ATR, ATRX, AXL, BARD1, BCL6, BLM, BMPR1A, BRAF, BRCA1, BRCA2, BTK, BUB1B, CARD11, CCND1, CCND3, CCNE1, CD79B, CDh2, CDK12, CDK4, CEP57, CFH, CREBBP, CSF1R, CTNNB1, DDR2, DIS3L2, DNMT3A, EGFR, EML4, EP300, EPCAM, ERBB2, ERBB3, ERCC1, ERCC2, ERCC4, ERCC5, ERG, ETS1, ETV4, EWSR1, EXT1, FANCA, FANCD2, FANCE, FANCG, FANCI, FANCM, FBXW7, FGF10, FGF2, FGF3, FGF6, FGFR1, FGFR3, FLCN, FLI1, FLT1, FLT3, FZR1, GATA2, GATA3, GEN1, GNA11, GNAS, HNF1A, HRAS, IDh2, IDh4, JAK1, JAK2, JAK3, KDR, KIT, KRAS, LDLR, MAGI1, MAP2K1, MAP2K2, MAX, MDM4, MED12, MET, MLh2, MLLT3, MMAB, MRE11, MSh4, MSH6, MTOR, NBN, NF1, NFE2L2, NOTCh1, NOTCh4, NOTCh6, NRAS, NRG1, NTRK1, NTRK3, PDGFRA, PDGFRB, PIK3CA, PIK3CD, PIK3CG, PIK3R1, PMS2, PPARG, PPP2R2A, PRKAR1A, PROC, PTCh2, PTPN11, RAD51B, RAD54L, RAF1, RB1, RBM45, RECQL4, RET, RHBDF2, ROS1, RPS6KB1, SDHB, SF3B1, SF3B2, SLTM, SLX4, SMARCB1, SMO, SMOX, STK11, TERT, TET2, TFRC, TP53, TP53BP1, TSC1, TSC2, WRN, XPA, XPC, ZNF395
Cosmics: 249
Allelic Frequencies: 1% - 92.5% for ddPCR-verified variants
Product Information
Verified Mutations:
Chromosome |
Gene |
Variant |
Expected Allelic Frequency % |
chr2 (29416025) |
ALK |
N/A (Ins) |
10.0% |
chr5 (112175770) |
APC |
p.T1493T |
35.0% |
chr7 (140453136) |
BRAF |
p.V600E |
10.7% |
chr13 (32913558) |
BRCA2 |
p.K1691fs*15 |
32.5% |
chr3 (41266101) |
CTNNB1 |
p.S33Y |
32.5% |
chr3 (41266133) |
CTNNB1 |
p.S45del |
10.0% |
chr7 (55241707) |
EGFR |
p.G719S |
24.5% |
chr7 (55249071) |
EGFR |
p.T790M |
0.90% |
chr7 (55259515) |
EGFR |
p.L858R |
2.80% |
chr7 (55242464) |
EGFR |
p.E746_A750 delELREA |
1.90% |
chr7 (55249063) |
EGFR |
p.Q787Q |
15.0% |
chr4 (153244155) |
FBXW7 |
p.S668fs*39 |
32.5% |
chr13 (28578214) |
FLT3 |
p.P986fs*>8 |
10.0% |
chr4 (55599321) |
KIT |
p.D816V |
10.0% |
chr4 (55602765) |
KIT |
p.L862L |
7.5% |
chr12 (25398281) |
KRAS |
p.G13D |
15.0% |
chr12 (25398284) |
KRAS |
p.G12D |
6.30% |
chr7 (116436022) |
MET |
p.A1357A |
7.0% |
chr7 (116339847) |
MET |
p.L238fs*25 |
7.0% |
chr9 (139409754) |
NOTCh1 |
p.P668S |
30.0% |
chr1 (115256530) |
NRAS |
p.Q61K |
12.5% |
chr3 (178936091) |
PIK3CA |
p.E545K |
8.80% |
chr3 (178952085) |
PIK3CA |
p.H1047R |
17.5% |
chr10 (43613843) |
RET |
p.L769L |
60.0% |
chr17 (7579472) |
TP53 |
p.P72R |
92.5% |
Unit Size: 1 FFPE section
Fixation: 4% PFA
Expected DNA yield: >400ng using Maxwell® 16 FFPE plus RSC DNA Purification Kit (Promega)
Storage: 4˚C
Expiry: See all product shelf life information
Quality Control
Allelic Frequency: Droplet Digital PCR™
Genotype: Next Generation Sequencing
Quality: Agarose gel electrophoresis
Quantification: QuantiFluor®
OncoSpan publications
Read these research articles citing the use of OncoSpan reference standards.
- Validation of a pan-cancer targeted next generation sequencing panel in New Zealand | New Zealand Journal of Medical Laboratory Science
- Enabling variant calling in challenging FFPE samples by coupling a novel library preparation chemistry with exome sequencing | Journal of Clinical Oncology
- Moving towards a local testing solution for undetermined thyroid fine-needle aspirates: validation of a novel custom DNA-based NGS panel | Journal of Clinical Pathology
- Performance Analysis of Three Bioinformatic Variant Callers Using a Somatic Reference Standard | Children's Mercy Kansas City
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If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order. We recommend that you use our online system to identify the products you require and their respective “HD” product codes.
To order offline we will require the following information:
- Product code(s) of the item(s) you wish to order (e.g. "HD123")
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- VAT Number (European Countries only)
If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please contact us.
Application notes
Certificate of analysis
Selection guides
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