Mimix™ PIK3CA E545K 50%, FFPE Reference Standard

The PIK3CA E545K Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of assays that detect somatic mutations, such as Sanger and qPCR sequencing assays.

DISCONTINUATION NOTICE: HD112 has been discontinued. This product has been replaced by HD200. For any further queries please contact us.

Horizon FFPE Reference standards are cell-line derived making them reproducible, renewable, and affordable experimental controls for molecular diagnostic assays. They are formulated to mimic patient samples and serve as excellent quality controls by acting as performance indicators for the whole diagnostic workflow.

 

Technical Information

Horizon FFPE standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. Using a proprietary method of mild fixation and homogenous paraffin embedding, we generate highly reproducible and consistent FFPE curls. Our standards yield high molecular weight DNA which can be analyzed in a wide-range of assays including qPCR, Sanger sequencing and next-generation sequencing.

 

Product Information

Product Description: PIK3CA E545K Reference Standard, 50%

Gene: PIK3CA

Amino Acid Change: E545K

Nucleotide change: c.1633G>A

Variant Allele Frequency: 50%

GRCh38 position: 3:179218303

Format: FFPE

Unit Size: 15 µm section

Fixation Method: 4% PFA (w/v)

Expected DNA Yield: ≥ 400ng Using Maxwell RSC Plus FFPE DNA Extraction kit

Storage: 4°C

Shipping: Ambient

Expiry: See all product shelf-life information

 

Quality Control

Genotype and Allelic Frequency: Droplet Digital PCRTM

DNA integrity: Agarose gel electrophoresis

Quantification: Quantifluor™

Intended use: For assay developers and molecular diagnostic labs for routine performance monitoring of molecular biology assays. Research use only. Not for diagnostic procedures.