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Tru-Q 1 (5% Tier) Reference Standard

The Tru-Q DNA Reference Standards are a highly-characterized, biologically-relevant quality control material used to assess the performance of NGS assays that detect somatic mutations.  The specifications of some variants within the Tru-Q product line have been changed for batches manufactured after the 31st March 2021. For more information, please click here.

Next generation sequencing (NGS) platforms give researchers much greater power when profiling tumor samples. However, the use of NGS presents challenges surrounding sample variability, platform bias, and potential failure to detect biomarkers. Horizon’s standards are appropriate for any NGS library preparation including whole-genome, whole-exome, custom capture and targeted amplicon panels. Horizon has developed this Quality-Seq (Q-Seq) NGS Reference Standard range to support the development and continued validation of Next Generation Sequencing platforms. The Tru-Q DNA Reference Standard portfolio covers multiple endogenous SNPs, insertions and deletions. Tru-Q NGS DNA Reference Standard 1 (5% Tier) covers 10 mutations at 5% allelic frequency in genomic DNA format. These may be diluted to even lower allelic frequencies using our Tru-Q 0 Wild Type standard. Furthermore, because the Tru-Q series has 4 different standards at the 5% alleleic frequency range, you may rotate these as “blinded” samples for proficiency testing in your laboratory. These standards are available only in gDNA format.

With this product you are able to:

  • Analyze the sensitivity and specificity of your assay
  • Gain certainty of the limit of detection and limit of quantification
  • Optimize and validate new cancer panels and routinely monitor the performance of your assay

Technical Data

Format: Genomic DNA

Genes Covered: BRAF, EGFR, FLT3, IDH1, JAK2, KRAS, MEK1 (MAP2K1), NOTCH1, NRAS, PIK3CA

Cosmics: 1,500+

Allelic Frequencies: 5% for verified variants

Buffer: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.1

 

Product Information

Verified Mutations: The Tru-Q Reference Standard has been manufactured using ten engineered cell lines and mixed to generate a 5% Allelic Frequency multiplex sample:

Gene Variant (AA) CDS mutation GRCh38 co-ordinates Cosmic ID Expected allelic frequency (%)
BRAF V600E c.1799T>A 7:140753336 COSV56056643 7.70
BRAF V600K c.1798_1799delinsAA 7:140753336-140753337 COSV56057713 3.80
EGFR G719S c.2155G>A 7:55174014 COSV51767289 16.70
EGFR T790M c.2369C>T 7:55181378 COSV51765492 4.20
FLT3 ΔI836 c.2508_2510del 13:28018500-28018502 COSV54043228 5.00
IDH1 R132C c.394C>T 2:208248389 COSV61615256 5.00
JAK2 V617F c.1849G>T 9:5073770 COSV67569051 5.00
KRAS G12A c.35G>C 12:25245350 COSV55497479 5.00
KRAS G12R c.34G>C 12:25245351 COSV55497582 5.00
KRAS G13D c.38G>A 12:25245347 COSV55497388 25.00
MEK1 (MAP2K1) P124L c.371C>T 15:66436825 COSV61068422 5.00
NOTCH1 L1600P c.4799T>C 9:136504892 COSV53025622 4.80
NRAS Q61K c.181C>A 1:114713909 COSV54736310 5.00
PIK3CA H1047R c.3140A>G 3:179234297 COSV55873195 30.00

Exome sequencing data: Download additional information on variants present in the parental cell lines

Unit Size: 1 µg

Concentration: 50 ng/µl

 

General Information

Storage: 4˚C

Expiry: See all product shelf life information

Cell Line Background: HCT116/RKO/SW48

 

Quality Control

Allelic Frequency: Droplet Digital PCR™

DNA Integrity: Agarose gel electrophoresis

DNA Quantification: Spectrophotometry (A260)