Tru-Q 3 (5% Tier) Reference Standard

The Tru-Q DNA Reference Standards are a highly-characterized, biologically-relevant quality control material used to assess the performance of NGS assays that detect somatic mutations. The specifications of some variants within the Tru-Q product line have been changed for batches manufactured after the 31st March 2021. For more information, please click here.

Next generation sequencing (NGS) platforms give researchers much greater power when profiling tumor samples. However, the use of NGS presents challenges surrounding sample variability, platform bias, and potential failure to detect biomarkers. Horizon’s standards are appropriate for any NGS library preparation including whole-genome, whole-exome, custom capture and targeted amplicon panels. Horizon has developed this Quality-Seq (Q-Seq) NGS Reference Standard range to support the development and continued validation of Next Generation Sequencing platforms. The Tru-Q DNA Reference Standard portfolio covers multiple endogenous SNPs, insertions and deletions. Tru-Q NGS DNA Reference Standard 1 (5% Tier) covers 10 mutations at 5% allelic frequency in genomic DNA format. These may be diluted to even lower allelic frequencies using our Tru-Q 0 Wild Type standard. Furthermore, because the Tru-Q series has 4 different standards at the 5% alleleic frequency range, you may rotate these as “blinded” samples for proficiency testing in your laboratory. These standards are available only in gDNA format.

With this product you are able to:

  • Analyze the sensitivity and specificity of your assay
  • Gain certainty of the limit of detection and limit of quantification
  • Optimize and validate new cancer panels and routinely monitor the performance of your assay

 

Technical Data

Format: Genomic DNA

Genes Covered: BRAF, EGFR, FLT3, GNA11, IDH1, KRAS, MET, NRAS, PIK3CA

Cosmics: 1,500+

Allelic Frequencies: 5% for verified variants

Buffer: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.1

 

Product Information

Verified Mutations: The Tru-Q Reference Standard has been manufactured using multiple engineered cell lines and mixed to generate multiple Allelic Frequencies in multiplex. This product has been verified by whole exome sequencing. The NGS determined allelic frequency and read depths are reported in the table below.

Gene Variant (AA) CDS mutation GRCh38 co-ordinates Legacy ID Genomic mutation ID Expected allelic frequency (%) NGS allelic frequency (%) NGS read depth
BRAF V600E c.1799T>A 7:140753336 COSM476 COSV56056643 7.70% 7.04% 866
BRAF V600M c.1798G>A 7:140753337 COSM1130 COSV56075762 3.80% 4.28% 865
EGFR G719S c.2155G>A 7:55174014 COSM6252 COSV51767289 16.70% 17.94% 680
EGFR ΔE746-A750 c.2235_2249del 7:55174771 COSM6223 COSV51765119 4.20% 1.88% 585
FLT3 D835Y c.2503G>T 13:28018505 COSM783 COSV54042116 5.00% 4.28% 957
GNA11 Q209L c.626A>T 19:3118944 COSM52969 COSV50017277 5.00% 4.51% 421
IDH1 R132H c.395G>A 2:208248388 COSM28746 COSV61615239 5.00% 3.39% 590
KRAS A146T c.436G>A 12:25225628 COSM19404 COSV55501778 5.00% 5.81% 534
KRAS G12S c.34G>A 12:25245351 COSM517 COSV55497461 5.00% 5.23% 555
KRAS G13D c.38G>A 12:25245347 COSM532 COSV55497388 25.00% 20.75% 559
MET Y1253D c.3757T>G 7:116783374 COSM700 COSV59257036 3.60% 3.73% 1554
NRAS Q61H c.183A>T 1:114713907 COSM585 COSV54736991 5.00% 5.05% 852
PIK3CA E542K c.1624G>A 3:179218294 COSM760 COSV55873227 5.00% 4.37% 1397
PIK3CA H1047R c.3140A>G 3:179234297 COSM775 COSV55873195 30.00% 32.08% 1035

Unit Size: 1 µg

Concentration: 50 ng/µl

 

General Information

Storage: 4˚C

Expiry: See all product shelf life information

 

Quality Control

Allelic Frequency: Droplet Digital PCR™

DNA Integrity: Agarose gel electrophoresis

DNA Quantification: Spectrophotometry (A260)