Learn how to design and build a custom plasmid for HDR insertion in your CRISPR experiment

This video will walk you through how to use our Edit-R HDR Donor Designer to order the components necessary to build your own plasmid repair template for HDR insertion of fluorescent reporters (EGFP/mKate2) or a custom sequence to any genomic site that has been cut by CRISPR-Cas9.

In this video you will learn how to:

• Navigate our Donor Designer for plasmid design
• Use our tool to choose the best components for your HDR-mediated experiment
• Order DNA components and additional kit elements necessary for building your custom plasmid

Additional Resources

CRISPR Design Tool Workflow

• Prior to beginning the design of a plasmid donor, learn how to design a CRISPR guide RNA for your site of interest

HDR Donor Designer

• A brief description of how to design and assemble a finished HDR donor plasmid

Edit-R HDR Plasmid Donor Kit

• Kits contain the static components needed to construct your custom HDR donor plasmid