Video: Edit-R™ HDR DNA Donor Designer: plasmid design
Learn how to design and build a custom plasmid for HDR insertion in your CRISPR experiment
This video will walk you through how to use our Edit-R HDR Donor Designer to order the components necessary to build your own plasmid repair template for HDR insertion of fluorescent reporters (EGFP/mKate2) or a custom sequence to any genomic site that has been cut by CRISPR-Cas9.
In this video you will learn how to:
- Navigate our Donor Designer for plasmid design
- Use our tool to choose the best components for your HDR-mediated experiment
- Order DNA components and additional kit elements necessary for building your custom plasmid
Additional Resources
CRISPR Design Tool Workflow
- Prior to beginning the design of a plasmid donor, learn how to design a CRISPR guide RNA for your site of interest
HDR Donor Designer
- A brief description of how to design and assemble a finished HDR donor plasmid
Edit-R HDR Plasmid Donor Kit
- Kits contain the static components needed to construct your custom HDR donor plasmid