Human Accell 2.0 siRNA Libraries
Accell 2.0 siRNA libraries are designed to support high-throughput functional gene perturbation in difficult-to-transfect cells.
Using Accell self-delivery chemistry, these libraries enable siRNA screening without transfection reagents, viral vectors, or electroporation, helping streamline workflows in cell types that are often challenging to manipulate.
These siRNA libraries are available in collections spanning the human drug targets and key gene families; and are available in siRNA SMARTpools and set of four individual siRNA formats.
Accell™ 2.0 siRNA Libraries
Accell 2.0 siRNA libraries represent the next generation of self-delivering RNAi reagents, designed for effective gene silencing in difficult-to-transfect cell types without the need for transfection reagents. Sequences are realigned and redesigned against the latest reference transcriptome, supported by over two decades of siRNA bioinformatic design and RNA chemical synthesis expertise, to support transcriptomic coverage, and target specific gene knockdown across diverse cell types and screening applications.
Collections span drug targets and commonly studied gene families, enabling interrogation of hundreds to thousands of genes in both focused and broad discovery screens. Available in 96- or 384-well formats with a choice of siRNA pools or individual unique designs, these libraries support diverse screening workflows and are compatible with single-well analytical techniques including high content imaging, morphological profiling, reporter-based assays, and high-throughput transcriptomics.
Highlights
- Next-generation transcriptome alignment and redesign: Accell 2.0 siRNAs are re-annotated and redesigned according to the latest reference transcriptome for improved specificity, with curation to maximize isoform coverage and account for updated genomic coordinates and newly annotated genes.
- Recommended for neuronal, suspension, primary, and other difficult-to-transfect cell types.
- Proprietary sense strand chemical modification strategy promotes selective antisense/active strand loading into the RISC to reduce off-target effects and facilitates cellular internalization without the need for transfection reagents.
- Nuclease resistance modifications protect siRNA integrity in biological environments, supporting sustained gene silencing activity.
- Extended-duration knockdown with optimized continuous application.
- Available as SMARTpool reagents to minimize seed-mediated off-target signatures while maintaining on-target knockdown.
- Available in 96- or 384-well plate formats; Echo® Qualified 384-Well plates available upon request.
- Plates include space for control reagent inclusion.
- Available as cherry-pick libraries for custom gene selection and hit follow-up.
Experimental Considerations
- Accell siRNA works at a higher concentration than conventional siRNA; recommended 1 µM working concentration
- Delivery may be inhibited by the presence of BSA in serum. Optimization studies with serum-free media formulations (Accell Delivery Media) or < 2.5% serum in standard media is recommended
- Full-serum media can be added back after 48 hours of incubation. Optimal mRNA silencing is typically achieved by 72 hours or up to 96 hours for protein knockdown
Learn more about Accell 2.0 siRNA design features and benefits on the Accell 2.0 siRNA product page
Available pre-defined collections and approximate gene counts: (table will be filled when we have the gene-count information)
| Human Accell 2.0 siRNA libraries | Approximate number of genes |
|---|---|
| GPCRs | 470 |
| Ion Channels | 420 |
| Phosphatases | 310 |
| Proteases | 670 |
| Protein Kinases | 760 |
| Ubiquitin Enzymes | 750 |
| Transcription Factors | 1,560 |
| Drug Targets | 3,370 |
| Ubiquitin Conjugation Subset 1 | 90 |
| Ubiquitin Conjugation Subset 2 | 110 |
| Ubiquitin Conjugation Subset 3 | 390 |
| Apoptosis | 550 |
| Cell Cycle Regulation | 170 |
| Cytokine Receptors | 140 |
| Deubiquitinating Enzymes | 100 |
| DNA Damage Response | 240 |
| Epigenetics | 810 |
| Membrane Trafficking | 140 |
| Nuclear Receptors | 50 |
| Tyrosine Kinases | 90 |
Please note, the whole genome library contains the Druggable Genome collection which contains 8 discrete sub-collections (light blue), and all remaining protein coding genes annotated in the relevant reference genome.
