CRISPR Design Tool

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CRISPR Design Tools

Guide RNA design and ordering for all your research needs

No Guide RNA Found

To generate guide options adjust design parameters by launching Advanced Options below.

For help in setting design parameters please reach out to our Scientific Support team.

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Add any of our CRISPR portfolio reagents (CRISPRko, CRISPRa, CRISPRi) to your cart to see new pricing (up to 56% off) now including All-in-one lentiviral sgRNA formats in 2026

Power and flexibility of Edit-R CRISPR design tool
Horizon’s Edit-R CRISPR design tool generates guide RNAs for straightforward knockout experiments, as well as custom site-specific guides that target user-defined regions of a gene
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By assessing phenotypes for thousands of designs, we established standards for identifying target sites that are more likely to achieve functional protein knockout while retaining high levels of specificity

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Supports 30+ species, alternative nucleases beyond standard SpCas9, and can design guides not just in the protein-coding region but also for microRNAs, long non-coding RNAs, and intronic regions

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Custom guide designs can be made as a synthetic guide or packaged into any of our lentiviral vectors, including the All-in-one vector, which combines SpCas9 sequence and a gene-specific guide RNA for a single, ready to use reagent.

Know what guide RNA sequence you need?
Order Guide RNA
Know the exact DNA sequence you want to edit?
Design Guide RNA
Need help?CRISPR design tool FAQs
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