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Cell Lines For Studying Ubiquitin-Conjugating Enzymes

Ubiquitin is a small regulatory protein found in the majority of eukaryotic tissues and is added to lysine residues of proteins as a post-translational modification.

The addition of ubiquitin can impact on protein regulation in a number of ways including degradation, cellular localisation, protein interactions and activity. These modifications can have diverse effects on a number of major cellular processes such as apoptosis, cell cycle, immune regulation and DNA transcription and repair amongst many others.

Ubiquitin conjugating Enzymes Diagram

  • Ubiquitin is attached to lysine residues in the target protein by a series of 3 main reactions: Activation, performed by ubiquitin activating enzymes or E1’s
  • Conjugation, performed by ubiquitin-conjugating enzymes or E2’s
  • Ligation, performed by ubiquitin-ligases or E3’s

The manner in which ubiquitin is added determines the outcome for the protein. These modifications can include:

  • Monubiquitination, the addition of a single ubiquitin to one lysine residue in the protein
  • Multi-monoubiquitination, the addition of single ubiquitin molecules to multiple lysine residues in the target protein
  • Polyubiquitination, the addition of multiple ubiquitin residues to a single lysine residue in the protein in the form of a chain which can take a number of different conformations.

These modifications alter cellular process in different ways. For example, monubiquitination can impact on membrane trafficking; however, some forms of polyubquitination can result in a protein being targeted for degradation via the proteasome. The ubiquitin pathway has been implicated in a number of genetic disorders and diseases including cancer and neurological diseases which result from the build-up of protein aggregates such as; Alzheimer’s disease, Parkinson’s disease and Huntingdon’s disease.

Examples of popular knockout cell lines for studying Ubiquitin-Conjugating Enzymes

UBE2A UBE2E1 UBE2H UBE2Q1
UBE2B UBE2E2 UBE2J1 UBE2W
UBE2C UBE2E3 UBE2J2 UBE2Z
UBE2D1 UBE2F UBE2K
UBE2D2 UBE2G1 UBE2L6
UBE2D4 UBE2G2 UBE2O

Browse our ready-to-go cell models to find your ideal cell line for ubiquitin-conjugating enzymes research.

Simply choose a cell line background, then search for your gene of interest to view all available ready-to-go models.

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