Here we talk about the role of the Edit-R Pooled sgRNA Indexing PCR Primers and answer the most common questions about the Indexing PCR and Sequencing Primer kit.
50 result(s)
Demonstration of a repeatable pooled lentiviral sgRNA screen in primary human T cells
Gene editing by CRISPR has led to significant advances in immunotherapy development. Here we offer several tips for successful editing in T cells, including recommendations for cell handling, nucleofection, and analysis.
Read about the top 4 considerations when deciding between clones and pools
Technology from Horizon Discovery has been awarded Top Innovation of the Year by The Scientist Magazine for the past seven consecutive years.
Interested in prime editing? Learn about this new technique and how Horizon's customizable long oligo synthesis can help get you started.
Achieve DNA-free guaranteed gene editing with synthetic predesigned sgRNA reagents and libraries covering the entire human genome.
Choosing the right cell-based screen from the plethora of options available can quickly become a complicated decision process. Here, we review two of the major options: cell panel and functional genomic screening.
After all the hard work of editing your cell line, you want to have confidence in your new research model. So, how do you verify your cell line is what you expect it to be? Could a heterogeneous cell population be obscuring your editing effects? Is observed phenotype being caused by the targeted gene edit, or unintended off-target effects? Here we discus ways to add supporting data to validate your gene-engineering projects.
CRISPR-edited cell lines are a useful tool to validate your antibodies before you start an experiment. They ensure you are using high quality reagents, so you can be confident in your results. Here we explain how you can use our edited cell lines for validation, the challenges to be aware of and how we can help you overcome them.