T-cell receptors (TCRs) are crucial components of T cell immunity, enabling the detection and subsequent elimination of abnormal, cancerous and infected cells. Dysfunctional T cell receptor signaling can lead to various immune deficiency or autoimmune diseases. In cancer treatment the study and modification of T cell receptor signaling is important for CAR-T cell therapy as well as modulation of the tumor microenvironment (TME). Modulating T cell activation can be important to avoid rejection of organ or stem cell transplants by the immune response.
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With the requirement of thorough in vitro assays and high throughput screening technologies to ensure drug safety and efficacy, make sure you're fully ready for when you submit your IND application by assessing immunogenicity with an MLR assay
Learn about how T cell exhaustion poses a significant challenge in immuno-oncology and the way epigenetic mechanisms can help develop strategies for reinvigorating exhausted T cells.
Learn about the MLR assay and why it is an essential component of the compound screening pipeline for drug development.
Accell siRNA has been widely literature-cited for gene silencing in primary immune cells, which have historically been difficult to transfect. Learn more here.
A discussion of novel methodology and research in primary human monocytes recently published by the labs of Dr. Alex Marson and Dr. Nevan Krogan.
Can base editing move cancer cell therapy further towards off-the-shelf treatments?
Here we discuss a recent paper by Gisela Jimenez-Duran and colleagues highlighting how a whole genome CRISPR knockout screen, carried out by Horizon Discovery, has enabled identification of a pre-existing inhibitor that can regulate pro-inflammatory macrophages.
Demonstration of a repeatable pooled lentiviral sgRNA screen in primary human T cells
Gene editing by CRISPR has led to significant advances in immunotherapy development. Here we offer several tips for successful editing in T cells, including recommendations for cell handling, nucleofection, and analysis.