Learn about the MLR assay and why it is an essential component of the compound screening pipeline for drug development.
Accell siRNA has been widely literature-cited for gene silencing in primary immune cells, which have historically been difficult to transfect. Learn more here.
A discussion of novel methodology and research in primary human monocytes recently published by the labs of Dr. Alex Marson and Dr. Nevan Krogan.
Can base editing move cancer cell therapy further towards off-the-shelf treatments?
Here we discuss a recent paper by Gisela Jimenez-Duran and colleagues highlighting how a whole genome CRISPR knockout screen, carried out by Horizon Discovery, has enabled identification of a pre-existing inhibitor that can regulate pro-inflammatory macrophages.
Demonstration of a repeatable pooled lentiviral sgRNA screen in primary human T cells
Gene editing by CRISPR has led to significant advances in immunotherapy development. Here we offer several tips for successful editing in T cells, including recommendations for cell handling, nucleofection, and analysis.