CRISPR-edited cell lines are a useful tool to validate your antibodies before you start an experiment. They ensure you are using high quality reagents, so you can be confident in your results. Here we explain how you can use our edited cell lines for validation, the challenges to be aware of and how we can help you overcome them.
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CRISPR technology now allows genes and molecular pathways to be examined with greater definition. We look at how knockout cell lines, either together with gene rescue and replication of disease mutations or as an independent cell model, can be used to validate your research and extend your findings
Revealing the role of E3 ubiquitin ligases in DNA damage repair. One of the diverse new uses for the HAP1 cell line, one that has begun to draw significant attention, is in the field of DNA damage repair. A recent paper from Minoru Takata's group highlights this important application of this relatively new tool.
Cancer fusion genes are hybrid genes that produce abnormal proteins believed to catalyze further cancer growth and increase invasiveness. Here we highlight novel research using CRISPR gene editing to specifically target cancer fusion genes, which could offer many potential therapeutic applications.
A look at how a new method dubbed SHERLOCK adapts CRISPR-based technology to a diagnostic platform to allow mutation detections in DNA and RNA.
This blog explores how a selection of HAP1 knockout cell lines were used to confirm the involvement of DDR pathways in compounds mode of action, demonstrating opportunities for the exploitation of synthetic lethal interactions.
Nuclease resistance improves performance of synthetic CRISPR RNA with Cas9 mRNA
Review of a paper published in Nature .com, detailing analysis of CRISPR-Cas9 sensitivity (Drop-out) screening, which resulted in the development of a streamlined screening pipeline to evaluate fundamental aspects of functional genomic screening.
The scientists at Horizon Discovery have published a robust and precise approach to generating translocations. This advancement facilitates the generation of relevant cell line models for oncology research.
Much information about the role of specific genes in fundamental biological processes and the onset and progression of genetic disease has been gleaned by researchers having the ability to selectively alter the genomic composition of individual genes and study the consequences. This approach enables researchers to observe the effects of a specific mutation, SNP or deletion in combination with the added layers of regulation present within the cell, including post-translational modification, epigenetic changes associated with chromatin structure, and transcriptional mechanisms.