dCas9-VPR and sgRNA expression from a single vector for simplified delivery and efficient gene activation or overexpression
Easy, ready-to-use (thaw virus, add to cells)
Single transduction = shorter time to results
Ideal for difficult to transfect cells and low passage cells
Enrich with either puromycin resistance or EGFP fluorescence
Transduction-ready vectors eliminate cloning and in vitro transcription steps
To shop for CRISPRa All-in-one Lentiviral sgRNA, please select a Gene.
Easily generate a stable cell line by activating your gene of interest with the CRISPRmod CRISPR activation (CRISPRa) system. This system is a unique adaptation of the classical CRISPR-Cas9 gene editing system which utilizes a catalytically deactivated Cas9 (dCas9) that is fused to a tripartite VPR activator. When paired with a well-designed guide RNA that targets a gene at the promoter region, or transcriptional start site (TSS), it promotes transcriptional activation.