CRISPRmod CRISPRa lentiviral sgRNA non-targeting controls
Lentiviral sgRNA constructs bioinformatically designed and validated to not target any gene in human or mouse genomes
Negative control CRISPRa sgRNAs to establish experimental baselines and to distinguish sequence-specific biological effects from non-specific effects. Available as high-titer purified lentiviral particle and glycerol stock formats
CRISPRa lentiviral sgRNA non-targeting controls are recommended as negative controls for experiments using CRISPRa lentiviral sgRNAs in human and mouse cells. They are designed to have a minimum of three mismatches or gaps to all potential PAM-adjacent targets in human, mouse and rat genomes. When using these controls, changes in cellular viability or gene expression likely reflect nonspecific cellular responses that can be used as a baseline for comparison to cells treated with target-specific reagents.
Highlights
- CRISPRa guide RNA non-targeting control sequences are cloned into the same optimized lentiviral vector as CRISPRa gene targeting sgRNAs
- Proprietary alignment tools used to verify at least three mismatches or gaps to any potential target in human, mouse or rat genomes
- Available as high-titer purified lentiviral particles or glycerol stocks
