Literature Review - Base editing platform for simultaneous knock-in and multiple gene knockout
This literature review delves into an application of gene editing that diverges from the conventional paradigm.
Modular Pin-point™ base editing system facilitates highly efficient and precise nucleotide conversion with the potential for multiplex gene editing capabilities. The modularity of the system means we can offer a customizable, off-the-shelf system for base editing.
Pin-point base editing technology
Revvity's Pin-point base editing technology is a three-component system featuring a nickase Cas9 which recruits the effector deaminase through fusion to an aptamer-binding protein. The aptamer is linked to the guide RNA associated with the nuclease in an extended, chimeric RNA scaffold. The modular nature of effector recruitment means that nCas9 can be used for the knockout of one or more gene targets while simultaneously inserting a transgene.
Now available for your base editing applications, these required components
- Guide RNA with patented and proprietary aptamer
- Modified Cas9 - nickase Cas9 (nCas9)
- Deaminase fused to an aptamer binding protein
Benefits of Pin-point platform include:
MODULAR SYSTEM for
optimized research
HIGH EFFICIENCY
gene editing platform
MULTIPLEX EDITING
across several targets
IMPROVED SAFETY over
standard CRISPR-Cas9
VERSATILE
TECHNOLOGY for targeted editing
VALIDATED
PERFORMANCE in T cells and iPSCs
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Pin-point Base Editing mRNA
The Pin-point base editing mRNA system allows for precisely directed point mutation edits without inducing double stranded breaks or the need for homology directed repair.
Pin-point Synthetic sgRNA Non-targeting Controls
Non-targeting synthetic controls for evaluation of Pin-point base editing. Bioinformatically designed not to target any gene in the human genome.
Pin-point Synthetic sgRNA Validated Controls
Synthetic sgRNA controls to verify optimal parameters for precise gene editing without inducing DNA double-strand breaks.
Custom Pin-point Synthetic sgRNAs
Order the 20 nt targeting sequence of your choice for use with the Pin-point platform
Learn more about how to design custom Pin-point Synthetic sgRNAs
Design guidelines for introducing point mutations to cause protein knockout with base editing
Learn more about base-editing
Literature review
This literature review delves into an application of gene editing that diverges from the conventional paradigm.
Don’t Break My Strands
CRISPR-engineered cell therapies with multiple gene knockouts could be limited by double-strand breaks
Base Editing: A Strong Contender in Cell and Gene Therapy
For safer CRISPR-based therapies, consider base editing technology, which has the advantage of maintaining genomic integrity
CRISPR 2.0: Base Editing in the Groove
A series of exciting preclinical and animal model results show that base editing is making rapid strides toward the clinic
Viewpoints: Base Editing: Expanding the Gene Editing Toolbox
Fast-paced developments and progress in the field of molecular genetics have greatly expanded our understanding of genes and their function in health and disease.