Revvity's Pin-point base editing platform offers a unique and powerful approach for safe and more effective complex genetic engineering. This page is dedicated for our Pin-point base editing reagents that are available for research use only. The Pin-point™ base editing platform technology is also available for clinical or diagnostic study and commercialization under a commercial license from Revvity. To learn more about potential licensing and service options please visit revvity.com
Modular Pin-point™ base editing system facilitates highly efficient and precise nucleotide conversion with the potential for multiplex gene editing capabilities. The modularity of the system means we can offer a customizable, off-the-shelf system for base editing.
Pin-point base editing technology
Revvity's Pin-point base editing technology is a three-component system featuring a nickase Cas9 which recruits the effector deaminase through fusion to an aptamer-binding protein. The aptamer is linked to the guide RNA associated with the nuclease in an extended, chimeric RNA scaffold. The modular nature of effector recruitment means that nCas9 can be used for the knockout of one or more gene targets while simultaneously inserting a transgene.
Now available for your base editing applications, these required components
- Guide RNA with patented and proprietary aptamer
- Modified Cas9 - nickase Cas9 (nCas9)
- Deaminase fused to an aptamer binding protein
Benefits of Pin-point base editing platform include:
MODULAR SYSTEM for
optimized research
HIGH EFFICIENCY
gene editing platform
MULTIPLEX EDITING
across several targets
IMPROVED SAFETY over
standard CRISPR-Cas9
VERSATILE
TECHNOLOGY for targeted editing
VALIDATED
PERFORMANCE in T cells and iPSCs
Read publications about the Pin-point base editing platform:
Order Pin-point base editing reagents
Custom Pin-point Synthetic sgRNAs
Use our custom ordering tool and learn more about design guidelines to introduce point mutations to cause protein knockout with base editing
Pin-point Base Editing mRNA
The Pin-point base editing mRNA system allows for precisely directed point mutation edits without inducing double stranded breaks or the need for homology directed repair.
Pin-point Synthetic sgRNA Non-targeting Controls
Non-targeting synthetic controls for evaluation of Pin-point base editing. Bioinformatically designed not to target any gene in the human genome.
Pin-point Synthetic sgRNA Validated Controls
Synthetic sgRNA controls to verify optimal parameters for precise gene editing without inducing DNA double-strand breaks.
Learn more in our base-editing reagents focused application notes
Complex genome engineering with the Pin-point base editing system, even in sensitive cell types
An application note demonstrating complex genome engineering with the Pin-point base editing system.
Guidance for using unmodified versus 5-methoxyuridine (5moU) modified mRNAs with chemically synthesized sgRNAs
Optimized stem and immune cell editing with the Pin-point™ base editing platform
Designing and evaluating single guide RNAs for introducing protein knockout with the Pin-point base editing platform
In this application note we’ll walk you through designing a guide RNA spacer sequence for a base editing experiment and demonstrate our approach to evaluating several candidate guide RNAs to identify the best one for generating a functional knockout.