miRIDIAN microRNA Mimic Positive Controls are ideal for optimizing conditions for relevant, well-controlled microRNA modulation experiments. Both positive and negative control molecules are provided for gain-of-function microRNA studies.

Successful microRNA functional studies begin with optimization of the assay in each cell line and/or type of interest. To optimize experimental conditions, we offer both housekeeping and endogenous positive controls for gain-of-function experiments using miRIDIAN Mimics.

Highlights

• Validated microRNA Mimic that targets Aldolase A in human, mouse, and rat
• Same structure and design as experimental miRIDIAN microRNA Mimics
• Allows optimization of assay conditions by monitoring mimic function on an endogenous gene target (Aldolase A) with a conserved miR-122 binding site (see Figure 1, Supporting Data)
• Based upon the targeted activity of miR-122 on Aldolase A mRNA levels in cell lines, expressing low to moderate levels of miR-122

Applications

• Link the activity of a microRNA mimic to post-transcriptional regulation of its putative gene target
• Measure modulation of endogenous Aldolase A mRNA levels