miRIDIAN microRNA Mimic Positive Controls are ideal for optimizing conditions for relevant, well-controlled microRNA modulation experiments. Both positive and negative control molecules are provided for gain-of-function microRNA studies.
Successful microRNA functional studies begin with optimization of the assay in each cell line and/or type of interest. To optimize experimental conditions, we offer both housekeeping and endogenous positive controls for gain-of-function experiments using miRIDIAN Mimics.
- Validated microRNA Mimic that targets Aldolase A in human, mouse, and rat
- Same structure and design as experimental miRIDIAN microRNA Mimics
- Allows optimization of assay conditions by monitoring mimic function on an endogenous gene target (Aldolase A) with a conserved miR-122 binding site (see Figure 1, Supporting Data)
- Based upon the targeted activity of miR-122 on Aldolase A mRNA levels in cell lines, expressing low to moderate levels of miR-122
- Link the activity of a microRNA mimic to post-transcriptional regulation of its putative gene target
- Measure modulation of endogenous Aldolase A mRNA levels
miRIDIAN microRNA Mimic Endogenous Control leads to regulation of Aldolase A in human, mouse and rat cell lines
Many cells lines express low to moderate levels of miR-122. Aldolase A is a predicted target of miR-122, and the 3' UTR is conserved in human, mouse, and rat at the 8mer miR-122 predicted seed site. miRIDIAN microRNA Mimics designed to modulate endogenous miR-122 was transfected at 50 nM (Huh-7 at 40 nM) using DharmaFECT 1 into the indicated cell lines and assessed for their ability to decrease AldoA mRNA levels. AldoA down-regulation was determined using the Quantigene® branched DNA assay (Panomics) at 3 days (HepG2 at 5 days) post-transfection.
The miRIDIAN microRNA Mimic Housekeeping Positive Controls allow for the direct monitoring of housekeeping genes in a microRNA mimic experiment. Positive Control #1 targets PPIB (aka Cyclophilin B).
The miRIDIAN microRNA Mimic Housekeeping Positive Controls allows for direct monitoring of housekeeping genes in a microRNA mimic experiment. Positive Control #2 targets GAPD so experimental conditions may be optimized with a validated mimic control.