miRIDIAN microRNA Mimic Positive Controls are ideal for optimizing conditions for relevant, well-controlled microRNA modulation experiments. Both positive and negative control molecules are provided for gain-of-function microRNA studies.
Successful microRNA functional studies begin with optimization of the assay in each cell line and/or type of interest. To optimize experimental conditions, we offer both housekeeping and endogenous positive controls for gain-of-function experiments using miRIDIAN Mimics.
- Same structure and design as experimental miRIDIAN microRNA Mimics
- Target the 3' UTR (untranslated region) of the standard housekeeping genes, PPIB, or GAPDH (see Figure 1, Supporting Data)
- Allows a clean, straightforward, cleavage-based readout of mimic function
- Delivery and function can be evaluated to determine transfection conditions in each cell line/type
- Monitor the function of a mimic molecule at the mRNA level using standard transcript quantification methods, such as RT-qPCR
miRIDIAN microRNA Mimic Housekeeping Controls serve as optimization tools
Figure 1. | miRIDIAN microRNA Mimics designed to target the 3' UTR of either PPIB or GAPDH were transfected at 50 nM using DharmaFECT 1 into the indicated cell lines and assessed for their ability to decrease target mRNA levels. PPIB or GAPDH down-regulation was determined using the Quantigene® branched DNA assay (Panomics) at 48 hours post-transfection.
The miRIDIAN microRNA Mimic Housekeeping Positive Controls allow for the direct monitoring of housekeeping genes in a microRNA mimic experiment. Positive Control #1 targets PPIB (aka Cyclophilin B).
The miRIDIAN microRNA Mimic Endogenous Positive Control helps you monitor specific mimic effects on target protein levels in a validated endogenous assay, which is based upon the targeted activity of miR-122 on Aldolase A mRNA levels in cell lines.