SMARTchoice promoter selection plate
A novel tool that enables straightforward, qualitative assessment of promoters that actively drive expression in your cells of interest.
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Identify active promoters in adherent cells using the SMARTchoice Promoter Selection Plate
In a single matrixed experiment, the SMARTchoice Promoter Selection Plate enables the evaluation of seven different constitutive promoters at a range of multiplicities of infection (MOIs) in your cells of interest.
After transduction of cells, wells can be quickly and visually evaluated for TurboGFP intensity using fluorescence microscopy, high-content imaging or detection using a microplate reader. The most active promoter (with best TurboGFP expression) will provide the highest expression of your SMARTvector reagent in your cells. Then simply order SMARTvector reagent with your choice of promoter and reporter!
For inducible promoter selection please visit the SMARTchoice inducible controls page
Product Details
An arrayed plate of SMARTvector Non-targeting Controls (NTCs) with seven well-characterized constitutive cellular promoters driving the expression of TurboGFP (Evrogen, Moscow, Russia)
High-titer lentiviral particles are packaged, purified, and concentrated for consistent activity
Lentiviral particles are arrayed in a convenient 96- well format (see supporting data ) in duplicate serial dilutions so multiple MOIs can be tested.
Highlights
- Enables straightforward qualitative assessment of promoters that actively drive expression (Figure 2)
- Quickly evaluate TurboGFP intensity using a fluorescent microscope, a high content imager, or a microplate reader
- Improve shRNA or microRNA resulting in (Figure 3):
- Increased performance of shRNA-based silencing
- Increased potency of shMIMIC microRNA gene target down-regulation
