Identify active promoters in adherent cells using the SMARTchoice Promoter Selection Plate
In a single matrixed experiment, the SMARTchoice Promoter Selection Plate enables the evaluation of seven different constitutive promoters at a range of multiplicities of infection (MOIs) in your cells of interest.
After transduction of cells, wells can be quickly and visually evaluated for TurboGFP intensity using fluorescence microscopy, high-content imaging or detection using a microplate reader. The most active promoter (with best TurboGFP expression) will provide the highest expression of your SMARTvector reagent in your cells. Then simply order SMARTvector reagent with your choice of promoter and reporter!
For inducible promoter selection please visit the SMARTchoice inducible controls page
An arrayed plate of SMARTvector Non-targeting Controls (NTCs) with seven well-characterized constitutive cellular promoters driving the expression of TurboGFP (Evrogen, Moscow, Russia)
High-titer lentiviral particles are packaged, purified, and concentrated for consistent activity
Lentiviral particles are arrayed in a convenient 96- well format (see supporting data ) in duplicate serial dilutions so multiple MOIs can be tested.
- Enables straightforward qualitative assessment of promoters that actively drive expression (Figure 2)
- Quickly evaluate TurboGFP intensity using a fluorescent microscope, a high content imager, or a microplate reader
- Improve shRNA, microRNA or Cas9 expression, resulting in (Figure 3):
- Increased performance of shRNA-based silencing
- Increased potency of shMIMIC microRNA gene target down-regulation
- Increased efficiency of Cas9-based editing
The SMARTchoice Promoter Selection Plate Layout
The SMARTchoice Promoter Selection Plate enables straightforward qualitative assessment of promoters that actively drive expression
Legend: Human A549, HEK293T and Jurkat cells were transduced with concentrated lentiviral particles arrayed in the SMARTchoice Promoter Selection Plate. TurboGFP expression was assessed by fluorescence microscopy 72 hours post-transduction. Images clearly demonstrate that the most functional promoter in A549 cells is mCMV, whereas the hCMV promoter is most active in HEK293T, and the mEF1α promoter is most active in Jurkat cells. TU = transducing unit.
Select the optimal promoter for maximum shRNA and shMIMIC expression
|Shipping Condition||Dry Ice|
|Storage Conditions||-80 C|
|Stability at Recommended Storage Conditions||At least 6 months|