The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. The encoded protein is found as a homodimer and is a member of the syndecan proteoglycan family. This gene is found on chromosome 20, while a pseudogene has been found on chromosome 22. [provided by RefSeq, Jul 2008].
ON-TARGETplus siRNA
siRNA designed and modified for greater specificity
- siRNA designed for higher specificity and reliable gene silencing, featuring patented chemistries that reduce off‑target activity. Available as single siRNA or in SMARTpool™ format for human, mouse, or rat.
- Guaranteed gene silencing
- Patented modifications to reduce off-targets
- Available as individual siRNA or in SMARTpool™ format – for human, mouse or rat
Knockdown your target gene function with confidence
The patented modification pattern for specificity, combined with the SMARTselection algorithm for efficient, guaranteed target gene silencing, makes Dharmacon ON-TARGETplus siRNA the premium choice for optimal knockdown and reduced off-targets.
Available as pre-designed, genome-wide reagents for human, mouse and rat.
- For the most up-to-date designs explore ON-TARGETplus 2.0 siRNA with continuous transcriptome alignment to maximize specificity and transcript isoform coverage
- Off-targets reduced by up to 90% compared to unmodified siRNA
- Guaranteed silencing with our SMARTpool™ format and 3 of 4 individual siRNAs (see Guarantee tab)
- Sequence information provided with siRNA purchase
ON-TARGETplus technology
A two-stranded mechanism requires a two-stranded solution.
Dharmacon scientists and collaborators demonstrated in 2006 (see Reference 1) that siRNA off-targets are mediated by antisense seed-region interactions, initiating the development of a dual-strand modification pattern:
- Sense strand is modified to prevent interaction with RISC and favor antisense strand uptake
- Antisense strand seed region is modified to destabilize off-target activity and enhance target specificity
Seed-region analysis on siRNA designs reduces miRNA-induced off-targets
A landmark publication (see Reference 2) from the Dharmacon research group was the first to experimentally demonstrate the key role of the seed region in mediating off-targets. A subsequent 2008 paper (see Reference 3) showed the importance of seed frequency in the 3' UTR as an indicator of its likelihood to cause off-targets. These principles were subsequently applied to our siRNA designs to improve specificity:
- Design filters eliminate common seed regions likely to cause miRNA-like off-targets
- Seed frequency analysis for siRNA designs minimize off-target effects
Product formats
- SMARTpool: A mixture of 4 siRNA provided as a single reagent; providing advantages in both potency and specificity.
- Set of 4: A convenient option for purchasing aliquots of all 4 individual siRNAs targeting a single gene.
- Individual siRNAs: For the most precise knockdown of specific transcript isoforms
Components for an RNAi experiment using synthetic siRNA
- ON-TARGETplus siRNA
- Appropriate positive and non-targeting controls
- DharmaFECT transfection reagent or electroporation for delivery
- siRNA buffer and/or RNase-free water for resuspension of siRNA.
Note: You can order guide RNA above, with the option to add any of these additional components directly to your cart.
Order quantity guidelines
ON-TARGETplus reagents are routinely used at 5 to 25 nM concentration. The calculations below, based on 25 nM, are for estimation purposes only and assume no loss from pipetting.
| Approximate # reactions (wells) at 25 nM siRNA concentration | |||
|---|---|---|---|
| nmol | 96-well plate (100 µL total reaction volume) | 24-well plate (500 µL total reaction volume) | 12-well plate (1000 µL total reaction volume) |
| 1 | 400 | 80 | 40 |
| 2 | 800 | 160 | 80 |
| 5 | 2000 | 400 | 200 |
| 10 | 4000 | 800 | 400 |
| 20 | 8000 | 1600 | 800 |
Custom siRNA design
Design and order custom siRNA sequences using our siDESIGN center.
