Transfection reagents & buffers

Efficient and reliable transfection with minimal cellular toxicity

 

Ensure proper RNA delivery with DharmaFECT™ low-toxicity transfection reagents.

DharmaFECT transfection reagents

For efficient siRNA & CRISPR guide RNA delivery

Optimized solutions for RNA transfection into a wide range of cell types for successful gene editing experiments.

Ancillary buffers & media

High quality buffers & media

Complete your experiment with all necessary ancillary reagents, including buffers, water, and specialized cell culture media.

Viral packaging

Generate your own lentiviral particles

Efficiently generate replication-incompetent lentiviral particles to deliver and express an shRNA or ORF construct into mammalian cells.

Pro tips for a successful transfection

Ensure successful RNA deliver every time

Read our top suggestions for transfection success! See how to select the right reagents for your needs and optimize conditions to achieve the best possible results.

Transfecting CRISPR guide RNA into stable Cas9 expressing cells

A straightforward workflow for CRISPR gene knockout

This application note describes optimizing the reverse transfection of synthetic guide RNA into a Cas9-expressing cell line using DharmaFECT™ transfection reagents

Co-transfecting Cas9 mRNA & synthetic CRISPR guide RNA

Quick protocol for CRISPR gene knockout

An abbreviated protocol for transfecting Edit-R™ synthetic guide RNA and Cas9 mRNA into cultured mammalian cells using DharmaFECT™ Duo transfection reagent

Co-transfecting Cas9, guide RNA & HDR donor template

Quick protocol for CRISPR based HDR mediated knock-in

A protocol for transfecting Edit-R™ synthetic guide RNA and Cas9 mRNA with an HDR donor template into cultured mammalian cells using DharmaFECT™ Duo transfection reagent