DharmaFECT transfection reagents
Optimized solutions for RNA transfection into a wide range of cell types for successful gene editing experiments
Ancillary buffers & media
Complete your experiment with all necessary ancillary reagents, including buffers, water, and specialized cell culture media
Viral packaging
Efficiently generate replication-incompetent lentiviral particles to deliver and express an shRNA or ORF construct into mammalian cells
Simultaneous delivery of CRISPR guide RNA and Cas9 DNA plasmid
This video demonstrates co-delivery of Edit-R™ CRISPR guide RNA along with a Cas9 expressing DNA plasmid.
Pro tips for a successful transfection
Read our top suggestions for transfection success! See how to select the right reagents for your needs and optimize conditions to achieve the best possible results.
Transfecting CRISPR guide RNA into stable Cas9 expressing cells
This application note describes optimizing the reverse transfection of synthetic guide RNA into a Cas9-expressing cell line using DharmaFECT™ transfection reagents
Knockout protocol: Co-transfecting Cas9 mRNA & synthetic CRISPR guide RNA
An abbreviated protocol for transfecting Edit-R™ synthetic guide RNA and Cas9 mRNA into cultured mammalian cells using DharmaFECT™ Duo transfection reagent
Knock-in protocol: Co-transfecting Cas9, guide RNA & HDR donor template
A protocol for transfecting Edit-R™ synthetic guide RNA and Cas9 mRNA with an HDR donor template into cultured mammalian cells using DharmaFECT™ Duo transfection reagent