Viral Packaging

Trans-lentiviral packaging system efficiently generates replication-incompetent lentiviral particles to deliver and express an shRNA or ORF (open reading frame) construct in either dividing or non-dividing mammalian cells.

Trans-lentiviral shRNA packaging kits
  • Trans-lentiviral shRNA packaging system

    Package your shRNA viral vector constructs into high-titer lentiviral particles for efficient transduction and gene silencing in the cell or tissue type of interest.

  • Trans-lentiviral ORF packaging system

    Package your ORF viral vector constructs into high-titer lentiviral particles for efficient transduction and gene expression in the cell or tissue type of interest.

References
  1. Naldini L., Blomer U., Gallay P., Ory D., Mulligan R., Gage F.H., Verma I.M., Trono D. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science, 1996; Apr 12;272(5259):263-7.
  2. Wu X., Wakefield J.K., Liu H., Xiao H., Kralovics R., Prchal J.T., Kappes J.C. Development of a novel trans-lentiviral vector that affords predictable safety. Mol Ther. 2000; 2(1):47-55.
  3. Kappes J.C., Wu X. Safety considerations in vector development. Somat Cell Mol Genet. 2001; 26(1-6):147-58.
  4. Kappes J.C., Wu X., Wakefield J.K. Production of trans-lentiviral vector with predictable safety. Methods Mol Med. 2003; 76:449-65.