Transfection reagents & buffers

Efficient and reliable transfection with minimal cellular toxicity

DharmaFECT transfection reagents

Optimized solutions for RNA transfection into a wide range of cell types for successful gene editing experiments

Ancillary buffers & media

Complete your experiment with all necessary ancillary reagents, including buffers, water, and specialized cell culture media

Viral packaging

Efficiently generate replication-incompetent lentiviral particles to deliver and express an shRNA or ORF construct into mammalian cells

Simultaneous delivery of CRISPR guide RNA and Cas9 DNA plasmid

This video demonstrates co-delivery of Edit-R™ CRISPR guide RNA along with a Cas9 expressing DNA plasmid.

Pro tips for a successful transfection

Read our top suggestions for transfection success! See how to select the right reagents for your needs and optimize conditions to achieve the best possible results.

Transfecting CRISPR guide RNA into stable Cas9 expressing cells

This application note describes optimizing the reverse transfection of synthetic guide RNA into a Cas9-expressing cell line using DharmaFECT™ transfection reagents

Knockout protocol: Co-transfecting Cas9 mRNA & synthetic CRISPR guide RNA

An abbreviated protocol for transfecting Edit-R™ synthetic guide RNA and Cas9 mRNA into cultured mammalian cells using DharmaFECT™ Duo transfection reagent

Knock-in protocol: Co-transfecting Cas9, guide RNA & HDR donor template

A protocol for transfecting Edit-R™ synthetic guide RNA and Cas9 mRNA with an HDR donor template into cultured mammalian cells using DharmaFECT™ Duo transfection reagent