OncoSpan gDNA
OncoSpan is the largest and most extensive oncology Reference Standard to date, featuring 386 variants across 152 key cancer genes.
In an exciting new first for Horizon, OncoSpan is provided with batch-specific NGS data, giving you knowledge and confidence of the background genotype of this cell-line-derived Reference Standard. OncoSpan’s larger gene list and better characterisation empowers users to perform more comprehensive validation of large oncology gene panels and exome sequencing assays than ever before.
OncoSpan is a well-characterised, cell line-derived Reference Standard containing 386 variants across 152 key cancer genes. This includes 249 variants with a COSMIC ID and 30 INDELs (24 deletions and 6 insertions, ranging from 2-16 base pairs). Variants are present between 1-100% allelic frequency (AF), with 52 variants present at ≤ 20% AF for LOD determination of your assay. Every batch of OncoSpan DNA has 25 variants confirmed by ddPCR, in addition to being fully exome sequenced by GATC-Eurofins at 500x coverage using Agilent SureSelect Human All Exon V6 kit and Illumina sequencing to ISO 17025. This provides you with an accurate and reliable truth set for comparison to your assay’s performance. You will receive a password upon ordering to download the raw NGS data for your batch (bam, bed and vcf files) via our File Transfer Protocol (FTP) portal.
Technical Data
Format
Genomic DNA
Genes Covered
ABL1, AKT1, AKT2, ALK, APC, AR, ARID1A, ATR, ATRX, AXL, BARD1, BCL6, BLM, BMPR1A, BRAF, BRCA1, BRCA2, BTK, BUB1B, CARD11, CCND1, CCND3, CCNE1, CD79B, CDH1, CDK12, CDK4, CEP57, CFH, CREBBP, CSF1R, CTNNB1, DDR2, DIS3L2, DNMT3A, EGFR, EML4, EP300, EPCAM, ERBB2, ERBB3, ERCC1, ERCC2, ERCC4, ERCC5, ERG, ETS1, ETV4, EWSR1, EXT1, FANCA, FANCD2, FANCE, FANCG, FANCI, FANCM, FBXW7, FGF10, FGF2, FGF3, FGF6, FGFR1, FGFR3, FLCN, FLI1, FLT1, FLT3, FZR1, GATA2, GATA3, GEN1, GNA11, GNAS, HNF1A, HRAS, IDH1, IDH2, JAK1, JAK2, JAK3, KDR, KIT, KRAS, LDLR, MAGI1, MAP2K1, MAP2K2, MAX, MDM4, MED12, MET, MLH1, MLLT3, MMAB, MRE11, MSH2, MSH3, MSH6, MTOR, NBN, NF1, NFE2L2, MOTCH1, NOTCH2, NOTCH3, NRAS, NRG1, NTRK1, NTRK3, PDGFRA, PDGFRB, PIK3CA, PIK3CD, PIK3CG, PIK3R1, PMS2, PPARG, PPP2R2A, PRKAR1A, PROC, PTCH1, PTPN11, RAD51B, RAD54L, RAF1, RB1, RBM45, RECQL4, RET, RHBDF2, ROS1, RPS6KB1, SDHB, SF3B1, SF3B2, SLTM, SLX4, SMARCB1, SMO, SMOX, STK11, TERT, TET2, TFRC, TP53, TP53BP1, TSC1, TSC2, WRN, XPA, XPC, ZNF395
Cosmics
249
Allelic Frequencies
1% - 92.5% for ddPCR-verified variants
Buffer
Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.1
Product Information
Verified Mutations
Chromosome |
Gene |
Variant |
Expected Allelic Frequency % |
chr2 (29416025) |
ALK |
N/A (Ins) |
10.0% |
chr5 (112175770) |
APC |
p.T1493T |
35.0% |
chr7 (140453136) |
BRAF |
p.V600E |
10.5% |
chr13 (32913558) |
BRCA2 |
p.K1691fs*15 |
32.5% |
chr3 (41266101) |
CTNNB1 |
p.S33Y |
32.5% |
chr3 (41266134) |
CTNNB1 |
p.S45del |
10.0% |
chr7 (55241707) |
EGFR |
p.G719S |
24.5% |
chr7 (55249071) |
EGFR |
p.T790M |
1.0% |
chr7 (55259515) |
EGFR |
p.L858R |
3.0% |
chr7 (55242465) |
EGFR |
p.E746_A750 delELREA |
2.0% |
chr7 (55249063) |
EGFR |
p.Q787Q |
15.0% |
chr4 (153244156) |
FBXW7 |
p.S668fs*39 |
32.5% |
chr13 (28578215) |
FLT3 |
p.P986fs*>8 |
10.0% |
chr4 (55599321) |
KIT |
p.D816V |
10.0% |
chr4 (55602765) |
KIT |
p.L862L |
7.5% |
chr12 (25398281) |
KRAS |
p.G13D |
15.0% |
chr12 (25398284) |
KRAS |
p.G12D |
6.0% |
chr7 (116436022) |
MET |
p.A1357A |
7.0% |
chr7 (116339848) |
MET |
p.L238fs*25 |
7.0% |
chr9 (139409754) |
NOTCH1 |
p.P668S |
30.0% |
chr1 (115256530) |
NRAS |
p.Q61K |
12.5% |
chr3 (178936091) |
PIK3CA |
p.E545K |
9.0% |
chr3 (178952085) |
PIK3CA |
p.H1047R |
17.5% |
chr10 (43613843) |
RET |
p.L769L |
60.0% |
chr17 (7579472) |
TP53 |
p.P72R |
92.5% |
Unit Size
1µg
Concentration
50ng/µl
General Information
Storage
4˚C
Expiry
See all product shelf life information
Quality Control
Allelic Frequency
Droplet Digital PCR™
Genotype
Next Generation Sequencing
Quality
Agarose gel electrophoresis
Quantification
Spectrophotometry (A260)
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