The Mammalian Gene Collection (MGC) is a trans-NIH initiative, started in 2001 and completed by the NIH in 2009. The MGC is now recognized as the gold standard for full-length, fully sequenced cDNA collections.
Its goal is to provide researchers with unrestricted access to sequence-validated full-length protein-coding (FL-CDS) cDNA clones for human, mouse, and rat genes. In 2005, the project added the bovine cDNAs generated by Genome Canada.
MGC clones were initially obtained by random EST-screening of cDNA libraries. Starting in 2004, MGC used transcript-specific RT-PCR cloning to isolate cDNAs for genes not represented in MGC. Finally, between 2006 and 2009, MGC employed DNA synthesis methods to provide FL-CDS clones for human and mouse genes still absent from the collection. Details on the vectors and methods employed at each stage of the MGC program are provided on the MGC website: Vectors & Method Overviews.
Human, mouse, rat, and bovine cDNAs from the Mammalian Gene Collection (MGC)
Human Expression Ready MGC cDNA Libraries are available as pre-defined gene families of clones in pCMV-SPORT6 expression vectors.
Human Mammalian Gene Collection (MGC) cDNAs arrayed in pre-defined gene family libraries.