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- Mimix™ Colorectal Cancer Panel FFPE Reference Standard
Mimix™ Colorectal Cancer Panel FFPE Reference Standard
Colorectal cancers (CRC) are the second leading cause of cancer-related mortality globally as of 2020. In the pursuit of diagnosis and personalized treatment strategies for CRC patients, molecular/genetic profiling plays a pivotal role. As with all the solid tumors, FFPE is the preferred choice of sampling to detect mutations which are diagnostically or prognostically relevant. Our CRC FFPE reference panel is meticulously blended using a unique combination of engineered cell lines harboring clinically significant mutations specific to CRCs. This FFPE panel replicates patient samples and serves as a robust reference material for validating NGS and other molecular biology methodologies aimed at detecting actionable mutations in key genes such as AKT1, APC, BRAF, KRAS, PIK3CA, and TP53 (refer to the accompanying table for a complete gene list). Like all Revvity Mimix reference standards, this product is manufactured in compliance with ISO 9001:2015 and ISO 13485:2016 standards.
Batch-specific supplementary NGS data
The Colorectal Cancer Panel FFPE Reference Standard comes with a batch specific supplementary NGS data. You can access the VCF file here. Each batch was sequenced using Agilent's CREV4 (Clinical Research Exome) kit at a depth of 250x on Illumina platform and run through the Illumina DRAGEN Somatic Pipeline for mapping, sorting, and variant calling.
Technical Data
Format: FFPE
Fixation Method: 4% PFA (w/v)
Section Size: 15µm
Cell Density: 3 x 108 cells per block. Approx. 3.5 x 105 cells per section
Expected DNA Yield: 400 ng using Promega Maxwell RSC FFPE Plus DNA Extraction kit
Genes covered: ABL1, ALK, AKT1, APC, BRAF, PIK3CA, KRAS, NRAS, PTEN, TP53
Allelic Frequencies: 3.00 - 22.00%
Verified Mutations:
Gene | DNA sequence change | Amino acid change | Expected allelic frequency (%) |
---|---|---|---|
ABL1 | c.1001C>T | p.T315I | 5.00 |
AKT1 | c.49G>A | p.E17K | 3.00 |
ALK | c.3522C>A | p.F1174L | 5.00 |
APC | c.8140C>T | p.R2714C | 22.00 |
BRAF | c.1799T>A | p.V600E | 4.00 |
KRAS | c.35G>A | p.G12D | 5.00 |
NRAS | c.181C>A | p.Q61K | 4.00 |
PIK3CA | c.1624G>A | p.E542K | 5.00 |
PTEN | c.388C>T | p.R130* | 5.00 |
TP53 | c.722C>T | p.S241F | 8.00 |
General information
Storage: 4°C
Shipping: Ambient
Expiration: See all product shelf life information
Quality control
Allelic frequency: Droplet Digital™ PCR
Quantification: Quantifluor
DNA Integrity: Agarose gel electrophoresis
Manufacturing Quality: ISO 9001:2015 and ISO 13485:2016
Intended use: For assay developers and molecular diagnostic labs for routine performance monitoring of molecular biology assays. Research use only. Not for diagnostic procedures.
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