MSI FFPE DNA Reference Standard
This MSI Reference standard contains 20 biomarkers relevant to determining the status of MSI in a patient sample, including markers in the National Cancer Institutes’ standardised Bethesda panel. Our reference standards are cell line-derived, for the closest representation of patient samples.
Microsatellites are repetitive sequences. Microsatellite Instability (MSI) relates to the misfunction of the DNA Mismatch Repair (MMR) gene that can introduce errors, in length or sequence, that result in novel repetitive sequences. MSI is most closely associated with colon cancers but shows prevalence in multiple other cancer types.
MSI is used in cancer diagnostics to aid the determination of an appropriate course of treatment for a patient. qPCR and NGS workflows are used to determine MSI status in patient samples. This standard has been shown to work with the Promega MSI kit, the Titano MSI kit, the Biocartis Idylla MSI kit and a laboratory developed test which utilises the Illumina MiSeq Sequencer.
Technical Data
Format
FFPE
Fixation Method
4% PFA
Section Size
15µm
Cell Density
250 million cells per 1cm core
Expected DNA Yield
400ng per section using Promega
Product Information
Verified Mutations
Verified as instable during QC: BAT-26, BAT-25, MONO-27, NR-21, NR-24. Confirmed as instable during development: BTBD7, DIDO1, ACVR2A, SEC31A, MRE11, SULF2, RYR3, BAT-40, TGFβ-11, D2S123, D18S58, D5S346, CAT-25, NR-22 and NR-27
Unit Size
1 section
Intended Use
Research Use Only
General Information
Allelic Frequency
No
Storage
2-8ᵒC
Quality Control
Quality
Biomarkers confirmed with Promega MSI Kit
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