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MSI FFPE DNA Reference Standard
This MSI Reference standard contains 20 biomarkers relevant to determining the status of MSI in a patient sample, including markers in the National Cancer Institutes’ standardised Bethesda panel. Our reference standards are cell line-derived, for the closest representation of patient samples.
Microsatellites are repetitive sequences. Microsatellite Instability (MSI) relates to the malfunction of the DNA Mismatch Repair (MMR) gene that can introduce errors, in length or sequence, that result in novel repetitive sequences. MSI is most closely associated with colon cancers but shows prevalence in multiple other cancer types.
MSI is used in cancer diagnostics to aid the determination of an appropriate course of treatment for a patient. qPCR and NGS workflows are used to determine MSI status in patient samples. This standard has been shown to work with the Promega MSI kit, the Titano MSI kit, the Biocartis Idylla MSI kit and a laboratory developed test which utilizes the Illumina MiSeq Sequencer.
Fixation Method: 4% PFA
Section Size: 15µm
Cell Density: 250 million cells per 1cm core
Expected DNA Yield: 400ng per section using Promega
Verified Mutations: Verified as instable during QC: BAT-26, BAT-25, MONO-27, NR-21, NR-24. Confirmed as instable during development: BTBD7, DIDO1, ACVR2A, SEC31A, MRE11, SULF2, RYR3, BAT-40, TGFβ-11, D2S123, D18S58, D5S346, CAT-25, NR-22 and NR-27
Unit Size: 1 section
Intended Use: Research Use Only
Allelic Frequency: No
Quality: Biomarkers confirmed with Promega MSI Kit
We accept orders using the following methods.
Our online catalog and ordering system can be used to search our full menu of available Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.
If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order. We recommend that you use our online system to identify the products you require and their respective “HD” product codes.
To order offline we will require the following information:
Product code(s) of the item(s) you wish to order (e.g. "HD123")
Your name, email address, title and organization
Shipping address and billing address with contact names
Purchase Order number if you have one
VAT Number (European Countries only)
Shipping information for all territories can be found on our Shipping Charges page. This includes Shipping and Handling fees, as well as shipping conditions for each product range.
If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please contact us.