This MSS Reference standard contains 20 biomarkers relevant to determining the status of MSI in a patient sample, including markers in the National Cancer Institutes’ standardised Bethesda panel. This matched microsatellite stable standard can be used as an additional control with our MSI standard.
MSI is used in cancer diagnostics to aid the determination of an appropriate course of treatment for a patient. qPCR and NGS workflows are used to determine MSI status in patient samples.
Our reference standards are cell line-derived, for the closest representation of patient samples.
Microsatellites are repetitive sequences. Microsatellite Instability (MSI) relates to the malfunction of the DNA Mismatch Repair (MMR) gene that can introduce errors, in length or sequence, that result in novel repetitive sequences. MSI is most closely associated with colon cancers but shows prevalence in multiple other cancer types.
MSI is used in cancer diagnostics to aid the determination of an appropriate course of treatment for a patient. qPCR and NGS workflows are used to determine MSI status in patient samples. This matched microsatellite stable standard can be used as an additional control with our MSI standard, and has been shown to work with the Promega MSI kit, the Titano MSI kit, the Biocartis Idylla MSI kit and a laboratory developed test which utilizes the Illumina MiSeq Sequencer.
Fixation Method: 4% PFA
Section Size: 15µm
Cell Density: 250 million cells per 1cm core
Expected DNA Yield: 400ng per section using Promega
Verified Mutations: Verified as stable during QC: BAT-26, BAT-25, MONO-27, NR-21, NR-24. Confirmed as stable during development: BTBD7, DIDO1, ACVR2A, SEC31A, MRE11, SULF2, RYR3, BAT-40, TGFβ-11, D2S123, D18S58, D5S346, CAT-25, NR-22 and NR-27
Unit Size: 1 section
Intended Use: Research Use Only
Allelic Frequency: No
Quality: Biomarkers confirmed with Promega MSI Kit
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