Mimix™ OncoPure Colorectal Cancer, FFPE reference standard
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Colorectal cancer (CRC) ranks as the second deadliest cancer worldwide, responsible for over 900,000 deaths annually. This figure is expected to increase by more than 80% in the next decade.*
Our Mimix™ OncoPure Colorectal Cancer FFPE reference standard offers a highly accurate representation of patient samples. It is derived from a single CRC tumor cell line without any modifications or engineering, providing a true reflection of a patient sample. With a consistent cell line background, this control enhances the reliability of your NGS and other molecular biology-based workflows to detect CRC.
Our Mimix reference standards are produced under ISO 13485 and undergo rigorous QC testing, leveraging over 14 years of experience. This means you can trust the quality of our controls and the accuracy of your results.
Batch-specific NGS data
The OncoPure Colorectal Cancer Panel FFPE Reference Standard comes with batch-specific NGS data. You can access the VCF file here. Each batch was sequenced using Agilent's CREV4 (Clinical Research Exome) kit at a depth of 250x on Aviti platform and analyzed using the Illumina DRAGEN Somatic Pipeline for mapping, sorting, and variant calling.
Technical Data
Format: FFPE
Section Size: 15µm
Cell Density: 1.5 x 108 cells per block. Approx. 4.5 x 105 cells per section
Expected DNA Yield: >400 ng using Promega Maxwell RSC FFPE Plus DNA Extraction kit
Genes covered: Please refer the VCF file for mutations
Allelic Frequencies: None claimed (refer to VCF file)
General information
Storage: 4°C
Shipping: Ambient
Expiration: See all product shelf life information
Quality control
Allelic frequency: Whole Exome Sequencing
Quantification: Quantifluor®
DNA Integrity: Agarose gel electrophoresis
Manufacturing Quality: ISO 9001:2015 and ISO 13485:2016
*Bray F, Laversanne M, Sung H, Ferlay J, Siegel RL, Soerjomataram I, Jemal A. Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2024 May-Jun;74(3):229-263. doi: 10.3322/caac.21834. Epub 2024 Apr 4. PMID: 38572751.
For research use only. Not for use in diagnostic procedures.
We accept orders using the following methods.
Online
Our online catalog and ordering system can be used to search our full menu of available Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.
Offline
If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order. We recommend that you use our online system to identify the products you require and their respective “HD” product codes.
To order offline we will require the following information:
- Product code(s) of the item(s) you wish to order (e.g. "HD123")
- Your name, email address, title and organization
- Shipping address and billing address with contact names
- Purchase Order number if you have one
- Telephone number
- VAT Number (European Countries only)
If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please contact us.
Safety data sheets
Selection guides
Reference Standards
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At what temperature do you ship reference standards?
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How can I open the NGS data .gz files?
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How do you manufacture your Horizon Reference Standards?
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How do you recommend extracting DNA from an FFPE reference standard?
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How was the batch-specific supplementary NGS data produced and analysed?
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Is it possible to order custom versions of Horizon Reference Standard?
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Is there a normal control in the same background and format as HD867, the Mimix OncoPure Colorectal Cancer Positive FFPE reference standard?
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The FFPE yield is lower than expected, how can I improve the yield?
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What batch-specific supplementary NGS data is provided?
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Where can I access batch-specific supplementary NGS data?
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Why do I see different allele frequencies or different variants in my NGS data when comparing to the data provided with the reference standard?