Mimix™ PIK3CA E542K 50%, gDNA Reference Standard

The PIK3CA E542K Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of assays that detect somatic mutations, such as Sanger and qPCR sequencing assays.

DISCONTINUATION NOTE: HD688 will be discontinued by 18-Apr-2025. Last order request date is on/before 08-Apr-2025. We are working to include this mutation in an upcoming PIK3CA multiplex panel which will be available from May/June 2025. In the meantime, you can use the following alternate product HD730. For any further queries please contact us. If you face any issues while placing the order, please reach out to orders@horizondiscovery.com or your local sales team or distributor.

Horizon DNA standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. The standard is provided at an allelic frequency of 50%, and may be diluted using the corresponding wild-type standard to generate a lower allelic frequencies for limit of detection studies. Horizon’s Base-Seq products are all derived from human cell lines, and are high molecular weight DNA. This format ensures that they may be applied to a wide-range of assays including qPCR, Sanger sequencing, next-generation sequencing, mass array, and more.

With this product you are able to:

  • Analyze the sensitivity of your assay
  • Gain certainty of the limit of detection
  • Optimize and validate new cancer panels and routinely monitor the performance of your assay

 

Technical Data

Synonyms: PI3Kα

Format: Genomic DNA

DNA Base Change: GAA→AAA

NCBI Reference Assembly SNP: rs121913273

Buffer: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0

 

Product Information

Unit Size: 1µg

Concentration: 50ng/µl

 

General Information

Allelic Frequency: 50%

Storage: 4˚C

Expiration: See all product shelf life information

 

Quality Control

Allelic Frequency: Droplet Digital PCR™

Genotype: Sanger sequencing of locus specific PCR

Quality: Agarose gel electrophoresis

Quantification: Spectrophotometry (A260)

Genotype: PIK3CA (E542K/+)

Intended use: For assay developers and molecular diagnostic labs for routine performance monitoring of molecular biology assays. Research use only. Not for diagnostic procedures.