Mimix™ PTEN Loss, FFPE Reference Standard
Phosphate and tensin homolog (PTEN) tumor suppressor gene is involved in the modulation of cell cycle progression by negatively regulating the PI3K-Akt signaling pathway which is responsible for cell survival and growth. The loss of PTEN gene (either partial or complete) leads to dysregulation of PI3K-Akt signaling pathway thereby leading to rapid cell growth and cancer progression. PTEN deletion is a prominent prognostic biomarker in prostate cancers along with AR gene copy number gain. Additionally, PTEN loss is also reported in several other pathologies like lung, ovary, endometrium, breast and colon cancers.
This FFPE reference standard contains a cell-line with homozygous deletion (copy number loss) of PTEN to mimic the loss of PTEN observed in several cancers. This product is manufactured in FFPE format under ISO 9001 and ISO 13485. This product will enable the development or monitoring of the efficacy of companion diagnostic tests manufactured to detect PTEN deletion.
Technical Product Information
Format: FFPE
Fixation method: 4% PFA (w/v)
Section size: 15µm
Cell density: 3 x 108 cells per block. Approx. 3.5 x 105 cells per section
Expected DNA yield: > 400 ng using Promega Maxwell RSC FFPE Plus DNA Extraction kit
Genes covered: PTEN
Verified mutations:
| Gene | DNA sequence change | Amino acid change | Expected copies |
|---|---|---|---|
| PTEN | Deletion | N/A | 0.00 |
General information
Storage: 4°C
Shipping: Ambient
Expiration: See all product shelf life information
Quality control
Allelic frequency: Droplet Digital™ PCR
Quantification: Quantifluor
DNA Integrity: Agarose gel electrophoresis
Manufacturing Quality: ISO 9001:2015 and ISO 13485:2016
Intended use: For assay developers and molecular diagnostic labs for routine performance monitoring of molecular biology assays. Research use only. Not for diagnostic procedures.
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Safety data sheets
Selection guides
Reference Standards
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At what temperature do you ship reference standards?
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Can the PTEN Loss FFPE Reference Standard HD864 be used to validate PTEN loss by NGS?
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How do you manufacture your Horizon Reference Standards?
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How do you recommend extracting DNA from an FFPE reference standard?
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Is it possible to order custom versions of Horizon Reference Standard?
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The FFPE yield is lower than expected, how can I improve the yield?
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What can be used by our customers as wildtype for HD864, the PTEN Loss FFPE Reference Standard?
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What is the importance of measuring PTEN loss and what is the relation of this marker with cancer progression?
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What is the specific deletion in the PTEN gene present in the PTEN Loss FFPE Reference Standard HD864?
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Which Droplet Digital PCR (ddPCR) system is used for variant detection at Horizon?