Mimix™ Quantitative Multiplex, FFPE (Moderate) Reference Standard

Revvity Mimix™ Quantitative Multiplex Moderate FFPE reference standard is a biologically relevant quality control material for assessing the performance of molecular biology assays that detect somatic mutations, such as qPCR, ddPCR, Sanger sequencing and NGS. Developed based on customer feedback, this product has more degraded DNA than the standard Quantitative Multiplex FFPE product (HD200). This allows for workflows to be tested with degraded samples that even more closely mimic patient samples in clinical and research setups.

For a limited time only, buy any 2 of the same Mimix reference standard products and get the 3rd FREE!*

*This promotion is only valid in China from 1st July 2025 to 30th September 2025 and on products of the same part number. The promotion cannot be combined with any other promotions, discounts, or offers. Conditions of the promotion may change, and Revvity may void, change, cancel, suspend, or amend this promotion without notice.

Patient-derived reference materials are variable and perishable, which creates complications for validation of workflows and assays. Revvity’s Mimix™ reference standards offer a source of consistent control material with patient-like performance, providing a solid foundation for monitoring and verifying research and diagnostic processes. Manufactured under ISO 9001 and ISO 13485, our cell line-derived reference material contains specific mutations at verified allelic frequencies, so that you can be more confident in your results.

Revvity Mimix™ standards are suitable for almost any NGS library preparation, including whole-genome, whole-exome, custom capture and targeted amplicon panels. The Quantitative Multiplex Reference Standard (QMRS) portfolio covers multiple endogenous SNPs, and deletions, in multiple formats - gDNA (HD701), FFPE (HD200, HD866) and Formalin-Compromised DNA (HD798, HD799, HD803). QMRS includes 11 highly onco-relevant mutations at allelic frequencies of 1%-24.5%.

The QMRS in FFPE format allows you to evaluate and optimize protocols from extraction to analysis, without wasting precious patient samples. As an extension to this, the moderately compromised FFPE provides a version that is more comparable to the degradation seen in real world samples.

With this product you can:

  • Evaluate workflow integrity from pre-analytical DNA extraction to post-analytical bioinformatics
  • Analyze the robustness of your workflow with moderately compromised FFPE format
  • Optimize and verify new cancer panels and monitor the performance of your assay with FFPE samples

 

Technical Product Information

Format: FFPE Moderate

Section Size: 15µm

Cell Density: 150 x 106 cells per block. Approx. 4.5 x 105 cells per section

Expected DNA Yield: > 200 ng using Promega Maxwell RSC FFPE Plus DNA Extraction kit

Genes Covered: BRAF, cKIT, EGFR, KRAS, NRAS and PIK3CA

Allelic Frequencies: 1.00 - 24.50%

 

Table 1: Verified Mutants and Expected Allelic Frequency

Gene

DNA sequence change

Amino acid change

Expected Frequency (%)

BRAF

c.1799T>A

p.V600E

10.50%

cKIT

c.2447A>T

p.D816V

10.00%

EGFR

c.2235_2249del

p.E746_A750del

2.00%

EGFR

c.2573T>G

p.L858R

3.00%

EGFR

c.2369C>T

p.T790M

1.00%

EGFR

c.2155G>A

p.G719S

24.50%

KRAS

c.38G>A

p.G13D

15.00%

KRAS

c.35G>A

p.G12D

6.00%

NRAS

 c.181C>A

p.Q61K

12.50%

PIK3CA

c.3140A>G

p.H1047R

17.50%

PIK3CA

c.1633G>A

p.E545K

9.00%

 

General Information

Storage: 4˚C

Shipping: Ambient

Expiration: See all product shelf life information

 

Quality Control

Allelic Frequency: Droplet Digital PCR™

Quantification: Quantifluor™

DNA Integrity: TapeStation Genomic DNA assay

Manufacturing Quality: ISO 9001:2015 and ISO 13485:2016.

Intended use: For assay developers and molecular diagnostic labs for routine performance monitoring of molecular biology assays. Research use only. Not for diagnostic procedures.