Dharmacon Edit-R™ CRISPR-Cas9 Gene Engineering System

When you need to knockout a gene next week, not next month

Edit-R requires no cloning and is ready-to-use!

The Edit-R CRISPR-Cas9 system allows you to very quickly complete your gene editing experiments by eliminating the time consuming process of cloning into an expression plasmid and subsequent sequencing to confirm proper insertion. Just provide the CRISPR Design Tool with a 20-base sequence targeting your DNA region of interest and the crRNA will be generated in a ready-to-use format. The Cas9 protein and tracrRNA are designed to interact and work with any crRNA generated by the CRISPR Design Tool.

The Edit-R CRISPR-Cas9 system includes the three critical components required for gene editing in mammalian cells, based on the natural S. pyogenes system:

  • a plasmid expressing a mammalian codon-optimized gene sequence encoding Cas9 nuclease
  • a 74-nucleotide tracrRNA
  • a custom crRNA designed to the target site of interest.

All three components are co-transfected into the mammalian cell of choice using the Dharmacon DharmaFECT Duo Transfection Reagent to perform gene knockout.

What does this mean for you? No more wasted time while cloning a sgRNA into a plasmid and waiting on sequencing results before you can start your experiment. Now you can get your results in a matter of days, not weeks.

Gene editing workflow using the Edit-R CRISPR Cas9 system

gene editing figure2 workflow

Additional Resources