Deubiquitinating Enzymes (DUBs)

Commonly used deubiquitinating enzymes genes

ATXN3 COPS6 CYLD JOSD2 OTUD3 OTUB1
OTUD5 PAN2 TDG
UNG
USP15 USP11
USP18 USP20 USP24
USP28
USP34 USP31
USP4 USP45 USP48
USP54
ZRANB1
USP8
BRCC3 COPS7A EIF3H
MPND
OTUD4
OTUB2
OTUD6B STAMBP UCHL1
USP1
USP16
USP13
USP19 USP21 USP25
USP3
USP38
USP32
USP40 USP46 USP49
USP6
USP9X

COPS5 COPS7B JOSD1
MYSM1
OTUD1

OTUD7B STAMBPL1 UCHL3
USP10
USP14

USP2

USP22

USP27X
USP30
USP33

USP42 USP47 USP53
USP7
VCPIP1

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Ubiquitination pathway

The ubiquitination pathway has been implicated in a number of genetic disorders and diseases including cancer and neurological diseases which result from the build-up of protein aggregates such as; Alzheimer’s disease, Parkinson’s disease and Huntingdon’s disease.

This collection has been generated in close collaboration with Prof. Steve Jackson (Gurdon Institute, Cambridge, United Kingdom).

Deubiquitinating Enzymes Diagram

Experimental validation

Deubiquitination of FANCD2 by USP1 is impacted in a USP1 knockout cell lines.

Cells were washed with PBS, lysed in Frackelton buffer (10 mM Tris/HCl pH 7.5, 50 mM NaCl, 30 mM sodium pyrophosphate, 1 % Triton X-100, 50 mM NaF and protease inhibitors).

They were analyzed by SDS-PAGE and Western blotting using anti-FANCD2 (sc-20022 from Santa Cruz). HAP1 wild-type cells were compared to cells bearing a frameshift mutation in USP1.

Deubiquitination image