The availability of our team to support you has not changed as a result of COVID-19. If there is a way we can assist you, we are here to help - Contact us

Deubiquitinating Enzymes (DUBs)

Ubiquitination pathway

The ubiquitination pathway has been implicated in a number of genetic disorders and diseases including cancer and neurological diseases which result from the build-up of protein aggregates such as; Alzheimer’s disease, Parkinson’s disease and Huntingdon’s disease.

This collection has been generated in close collaboration with Prof. Steve Jackson (Gurdon Institute, Cambridge, United Kingdom).

Deubiquitinating Enzymes Diagram

Experimental validation

Deubiquitination of FANCD2 by USP1 is impacted in a USP1 knockout cell lines.

Cells were washed with PBS, lysed in Frackelton buffer (10 mM Tris/HCl pH 7.5, 50 mM NaCl, 30 mM sodium pyrophosphate, 1 % Triton X-100, 50 mM NaF and protease inhibitors).

They were analyzed by SDS-PAGE and Western blotting using anti-FANCD2 (sc-20022 from Santa Cruz). HAP1 wild-type cells were compared to cells bearing a frameshift mutation in USP1.

Deubiquitination image

Examples of popular knockout cell lines for deubiquitinating enzymes genes

ATXN3 COPS6 CYLD JOSD2 OTUD3 OTUB1
OTUD5 PAN2 TDG UNG USP15 USP11
USP18 USP20 USP24 USP28 USP34 USP31
USP4 USP45 USP48 USP54 ZRANB1 USP8
BRCC3 COPS7A EIF3H MPND OTUD4 OTUB2
OTUD6B STAMBP UCHL1 USP1 USP16 USP13
USP19 USP21 USP25 USP3 USP38 USP32
USP40 USP46 USP49 USP6 USP9X
COPS5 COPS7B JOSD1 MYSM1 OTUD1
OTUD7B STAMBPL1 UCHL3 USP10 USP14
USP2

USP22

USP27X USP30 USP33
USP42 USP47 USP53 USP7 VCPIP1

Browse our ready-to-go cell models to find your ideal cell line for Deubiquitinating Enzymes (DUBs) research.

Simply choose a cell line background, then search for your gene of interest to view all available ready-to-go models.

Browse Our Cell Lines

Can't find the right cell model for your research?

Our Express and Custom engineering services could help you.

Find out more