Thanks to many years of research, scientists all around the world have started uncovering the function that long non-coding RNAs (lncRNAs) have in mammalian organisms. The added knowledge about lncRNAs has changed its classification from “junk” RNA to be the complete proof that our genome is fully functional.
Exploration of lncRNA suggests that it may function in many critical cellular processes, from chromatin remodeling at the DNA level to scaffolding at the protein-protein level, and beyond – revealing that our initial understanding was little more than the tip of an iceberg. However, as our knowledge increases, we need more tools to be able to parse out what is happening in our cells.
At Horizon Discovery, we have a variety of products available to support your research on this exciting topic. Some researchers start with our catalog siRNAs targeting human and mouse noncoding RNA genes in the RefSeq database (Lincode siRNA). Others choose predesigned control sequencesto match their experiment. Finally, fully custom RNA oligos allow even more strategies to downregulate candidate lncRNAs to fit specific needs.
Below is a helpful table summarizing the experimental methods from lncRNA peer reviewed publications using Horizon's siRNAs targeting long noncoding RNAs. We hope this information will spark ideas for how to perform experiments to explore yet unknown functions of your favorite lncRNA. Horizon Discovery is confident that our products will help you uncover more about the role of lncRNAs. Please reach out to our technical services group anytime for more information – we’d enjoy helping you get started with lncRNA studies.
|Publication title||Year||in vitro or in vivo||Catalog||Custom||Library||siRNA concentration||Cell /animal model|
|in vitro||X||X||Not mentioned||CaSki|
|2017||in vitro and in vivo||X||Not mentioned||Mouse/breast cancer brain metastasis (BCBM)|
|2019||in vitro||X||5 nM||HEK293|
|2015||in vitro||X||10 nM||Urothelial carcinoma (UC)|
|SAM68 is required for regulation of Pumilio by the NORAD long noncoding RNA||2018||in vitro||X||50 nM||U2OS and HCT116|
|ANRIL: A Regulator of VEGF in Diabetic Retinopathy||2017||in vitro||X||5 nM||Human retinal endothelial cells|
|A ROR1-HER3-LncRNA signaling axis modulates the Hippo-YAP pathway to regulate bone metastasis||2017||in vitro||X||15 pmol Lincode||MCF-7|
|LOC285629 regulates cell proliferation and motility in colorectal cancer cells||2017||in vitro||X||Not mentioned||SW480, CCD-112-CoN, COLO-205, HT-29|
|A conserved abundant cytoplasmic long noncoding RNA modulates repression by Pumilio proteins in human cells||2016||in vitro||X||50 nM and 100 nM||U2OS and HeLa|