CRISPRi dCas9-SALL1-SDS3 lentiviral particles express a human codon-optimized version of the nuclease-deactivated Cas9 gene, fused to proprietary transcriptional repressors (SALL1 and SDS3). When paired with a well-designed guide RNA that targets a gene near the native transcription start site, expression is repressed.

Review our CRISPRi applications page to get an overview of the technology!

Highlights of using dCas9-SALL1-SDS3 lentiviral particles

• Generate your own stable "CRISPRi ready" dCas9-SALL1-SDS3 expressing cell line.
• Concentrated, purified lentiviral particles are ready for immediate transduction (minimum ≥1×10⁷ TU/mL functional titer, by qPCR).
• Co-expression of blasticidin resistance gene allows for easy cell population selection or for clonal expansion of individual cells.
• Choose from one of three different promoters for optimum expression in your cells of interest (see options below).

Requirements for a CRISPRi experiment using dCas9-SALL1-SDS3 lentiviral particles

• CRISPRi dCas9-SALL1-SDS3 lentiviral particles
• CRISPRi lentiviral sgRNA for your target gene (see Workflow tab)

Not all RNA pol II promoters are equally active in different cellular environments

The activity of any given promoter controlling the transcription of dCas9-SALL1-SDS3 can differ greatly from one biological context to another, resulting in variable dCas9-SALL1-SDS3 expression levels and thus varying levels of gene repression. Choosing an optimal promoter for your cell line will be important for robust gene overexpression.

SMARTchoice promoter options for expressing dCas9-SALL1-SDS3