CRISPRmod CRISPRi lentiviral sgRNA
Predesigned CRISPRi lentiviral sgRNAs for highly efficient gene repression of any human gene
- An ideal delivery method for CRISPRi in difficult-to-transfect cells, or when longer term repression is desired
- Available as high-titer lentiviral particles or glycerol stock
CRISPRi lentiviral sgRNA
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Efficient endogenous gene repression with CRISPRi
CRISPR interference (CRISPRi) is a unique adaptation of the CRISPR-Cas9 gene editing system. The Horizon CRISPRmod CRISPRi system utilizes a catalytically deactivated Cas9 (dCas9) fused to our proprietary transcriptional repressors (SALL1 and SDS3). When paired with an algorithm-designed guide RNA that targets a gene immediately downstream of the transcriptional start site (TSS), it promotes transcriptional repression.
Review our CRISPRi applications page to get an overview of the technology!
Highlights of CRISPRi lentiviral sgRNA reagents
- Available as glycerol stocks or high-titer purified lentivirus particles
- Designed with a published algorithm by Horlbeck, et. al. (2016) that has demonstrated strong levels of gene repression from optimized designs
- Purified, concentrated high-titer lentivirus particles can be directly transduced into cells, including difficult-to-transfect cells and lines
Requirements for a CRISPRi experiment using lentiviral sgRNA
- CRISPRi lentiviral sgRNA for your target gene
- CRISPRi dCas9-SALL1-SDS3 lentiviral particles (see Workflow tab)
Schematic diagram of the CRISPRi lentiviral sgRNA vectors
In the CRISPRi lentiviral sgRNA vector backbone, the gene-specific guide RNA is expressed under the control of a human U6 promoter, while expression of the puromycin resistance gene (PuroR) is driven from the mouse CMV promoter and allows for rapid selection of cells with integrated sgRNA. The plasmid contains the AmpR resistance marker for growth and selection in E. coli.
