CRISPRi lentiviral sgRNA positive controls
Validated CRISPRi lentiviral sgRNA for optimization of transcriptional repression experiments
Verify optimal experimental conditions and ensure dCas9-SALL1-SDS3 expression for efficient gene repression
CRISPRi lentiviral sgRNA positive controls will selectively repress a well characterized human gene (either PPIB1 or SEL1L). Positive controls are recommended for optimization and ongoing monitoring of delivery conditions and dCas9-SALL1-SDS3 expression prior to experiments with target gene-specific guide RNAs.
Highlights
- Select PPIB1 or SEL1L as your positive control gene
- Available as glycerol stocks and high-titer purified lentiviral particles (50 µL at ≥ 1 x 108 TU/mL)
- Ideal for optimization of experimental conditions and confirming successful gene repression
In addition to positive controls, it is recommended to use a CRISPRi lentiviral sgRNA non-targeting control to determine baseline levels of gene expression.
