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CRISPRmod CRISPRi synthetic sgRNA non-targeting controls
Validated CRISPRi synthetic pools or individual sgRNA for evaluation of transcriptional repression experiments
Used to evaluate baseline cellular responses to CRISPRi components in the absence of gene target-specific sgRNA
CRISPRi synthetic sgRNA non-targeting controls are designed and recommended for use as negative controls for transcriptional repression experiments using CRISPRi sgRNA. These non-targeting controls will engage the dCas9-SALL1-SDS3 complex, but will not target any PAM-adjacent sites in the human genome. Any observed alteration in gene expression levels or viability in cells treated with these controls can be used as a baseline response of the cells to the dCas9-SALL1-SDS3 complex for comparison to those treated with target-specific CRISPRi synthetic sgRNA.
Highlights
- Proprietary alignment tools used to verify at least three mismatches or gaps to any potential target in the human genome
- Choose individual or pooled CRISPRi sgRNA controls to match your experimental CRISPRi sgRNA reagent format
- Ideal for establishing baseline levels of gene expression
In addition to non-targeting controls, it is recommended to use a CRISPRi synthetic positive control to optimize experimental conditions for efficient transcriptional repression.
