CRISPRmod CRISPRi synthetic sgRNA
Predesigned CRISPRi guide RNAs for highly specific gene repression of any human protein-coding gene
- Our CRISPRi system has been developed to outperform other systems (KRAB and MECP2) when combined with synthetic guides - observe repression within 24 hours post-transfection
- Co-transfect or electroporate with CRISPRi dCas9-SALL1-SDS3 mRNA for a lentiviral-free CRISPRi workflow
- Available as pooled or individual reagents
CRISPRi synthetic sgRNA
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Efficient transcriptional gene repression with CRISPRi
CRISPR interference (CRISPRi) is a unique adaptation of the CRISPR-Cas9 gene editing system. The Horizon CRISPRmod CRISPRi system utilizes a catalytically deactivated Cas9 (dCas9) fused to our proprietary transcriptional repressors (SALL1 and SDS3). When paired with an algorithm-designed guide RNA that targets a gene immediately downstream of the transcriptional start site (TSS), it promotes transcriptional repression.
Review our CRISPRi applications page to see an overview of the technology!
Highlights of CRISPRi synthetic sgRNA reagents
- CRISPR-based PAM-anchored targeting provides highly specific transcriptional repression (see Supporting data tab)
- Designs optimized from a published algorithm by Horlbeck, et. al. demonstrate strong levels of gene repression (see References tab)
- Chemically modified CRISPRi sgRNAs provide additional stability against nuclease degradation and improve overall performance
- For genes with alternative characterized start sites, distinct guide RNA designs are available (labeled P2)
Requirements for a CRISPRi experiment using synthetic sgRNA
- CRISPRi synthetic sgRNA for your target gene - choose from individual guides, sets of 3 guides, or pools of 3 guides
- CRISPRi dCas9-SALL1-SDS3 source - choose from mRNA or lentiviral particles (see Workflow tab)
- Appropriate positive and non-targeting controls
- DharmaFECT transfection reagent or electroporation for guide delivery
