Validated CRISPRi synthetic pools or individual sgRNA for optimization of transcriptional repression experiments
Used to verify optimal experimental conditions and ensure dCas9-SALL1-SDS3 expression for efficient gene repression
CRISPRi synthetic sgRNA positive controls will selectively repress a well characterized human gene (either PPIB1 or SEL1L). Positive controls are recommended for optimization and ongoing monitoring of delivery conditions and CRISPRi dCas9-SALL1-SDS3 expression prior to experiments with target gene-specific guide RNAs.
Select PPIB1 or SEL1L as your positive control gene
Choose individual or pooled CRISPRi sgRNA controls to match your experimental CRISPRi sgRNA reagent format
Ideal for optimization of experimental conditions and confirming successful gene repression
Robust gene knockdown with CRISPRi synthetic sgRNA positive controls
Robust transcriptional gene repression with co-delivery of dCas9-SALL1-SDS3 mRNA and CRISPRi synthetic sgRNA positive controls. K562 and Jurkat cells were nucleofected with dCas9-KRAB or dCas9-SALL1-SDS3 mRNA (2 µg), and pooled CRISPRi synthetic sgRNAs (5 µM) targeting PPIB and SEL1L via a Lonza 96-well Shuttle system. WTC-11 hiPS cells were nucleofected with dCas9-KRAB or dCas9-SALL1-SDS3 mRNA (1 µg) and pooled CRISPRi synthetic sgRNA (3 µM) targeting PPPIB or SEL1L via a Lonza 96-well Shuttle system. Cells were harvested 72 hours post-nucleofection. Total RNA was isolated, and relative gene expression was measured using RT-qPCR. The relative gene expression for each target gene was calculated with the Cq method using GAPDH as the housekeeping gene and normalized to a non-targeting control (NTC).