miRIDIAN microRNA Hairpin Inhibitor Negative Controls are ideal for optimizing conditions for relevant, well-controlled microRNA modulation experiments. Both positive and negative control molecules are provided for loss-of-function microRNA studies.
Figure 1. | Effects of miRIDIAN microRNA Hairpin Inhibitor Negative Controls on the function of miR-21 were assayed at 48 hours after transfection of 5 nM inhibitor or negative control in H9c2, NIh6T3, or HeLa cells using DharmaFECT Duo and a dual luciferase reporter system.
Choose from two universal miRIDIAN Hairpin Inhibitor Negative Controls for experiments with miRIDIAN microRNA Hairpin Inhibitors. Negative control sequences based on C. elegans microRNAs have minimal sequence identity in human, mouse, and rat.
miRIDIAN microRNA Hairpin Inhibitor Positive Control targets miR-16 for monitoring the effects of a microRNA inhibitor in a validated, endogenous assay, allowing for microRNA functional assays optimization and hairpin inhibitor function evaluation.
The miRIDIAN microRNA Hairpin Inhibitor Transfection Control is a Cy3-labeled microRNA Hairpin Inhibitor based on the C. elegans miRNA cel-miR-67 (miRIDIAN Hairpin Inhibitor Negative Control #1) for monitoring delivery into human, mouse, and rat cells.