miRIDIAN microRNA Hairpin Inhibitor Positive Control
A validated microRNA inhibitor designed to target miR-16 in human, mouse and rat
miRIDIAN microRNA Hairpin Inhibitor Positive Control targets miR-16 for monitoring the effects of a microRNA inhibitor in a validated, endogenous assay, allowing for microRNA functional assays optimization and hairpin inhibitor function evaluation.
The miRIDIAN microRNA Hairpin Inhibitor Positive Control is ideal for optimizing conditions for relevant, well-controlled microRNA modulation experiments. Both positive and negative control molecules are provided for loss-of-function microRNA studies.
Successful microRNA functional studies begin with optimization of the assay in each cell line and/or type of interest. To optimize inhibition experimental conditions by assessing the effect of inhibition on an endogenously expressed microRNA, we offer a validated Hairpin Inhibitor Positive Control.
Targets endogenous miR-16 in human, mouse, and rat cells resulting in reduced miR-16 levels (seeFigure 1, Supporting Data)
Optimize microRNA inhibition assays by targeting a microRNA in a cell line that expresses miR-16
Specific inhibition of miR-16 can be directly assessed by RT-qPCR and/or Northern blotting
Stability at Recommended Storage Conditions
At least 12 months
2 nM of miRIDIAN miR-16 Hairpin Inhibitor is sufficient to bind or sequester a majority of endogenous miR-16
Figure 1. | Endogenous levels of miR-16 were assayed 48 hours after transfection of either miRIDIAN miR-16 Hairpin Inhibitor, miR-21 Hairpin Inhibitor or Hairpin Inhibitor Negative Control #1. HeLa cells were transfected using DharmaFECT 1.