ON-TARGETplus™ siRNA
siRNA of choice for high-specificity and reduced off-target effects
Knockdown with confidence using a premium synthetic siRNA, chemically modified for maximum specificity.
RNA interference via synthetic small interfering RNA (siRNA) is the preferred choice for fast but reliable loss-of-function studies. Broadly useful across many applications and in any cell line, siRNA offers a straightforward and widely cited technique for functional gene silencing. For over 20 years, Dharmacon reagents have remained the most trusted name in high quality siRNA design and synthesis.
Select a predesigned siRNA oligonucleotide or design your own siRNA with desired chemical modifications.
Accell™ siRNA
Gene silencing in difficult cell types
Silence genes in difficult-to-transfect cell types without transfection or electroporation using self-delivering siRNA.
siGENOME™ siRNA
Our longest standing siRNA product
Reliable gene silencing from the longest standing siRNA guarantee on the market.
Our siRNA knockdown guarantee
The ON-TARGETplus, Accell and siGENOME siRNA lines are guaranteed* to effectively silence target gene expression by at least 75% at the mRNA level.
DharmaFECT™ transfection reagents
Ensure successful siRNA delivery
Optimized solutions for successful RNA transfection into a wide range of cell types.
siRNA controls
Complete your siRNA experiment
Appropriate controls for all of our RNAi product lines.
Not sure where to start?
Ask an RNAi expert
Need help planning an RNAi experiment, choosing a transfection reagent or finding proper controls? Our expert scientific support team is here to help!
Lincode™ siRNA
For studies focused on lncRNA
Knockdown long, non-coding RNA genes with specialized siRNA, modified to ensure specificity.
Custom siRNA design
Use our siDESIGN Center to create a custom siRNA to any gene for any species or provide your own sequence for custom modified siRNA synthesis
Reducing off-targets maximizes specificity
A successful siRNA knockdown experiment must be both effective and specific. Horizon’s SMARTselection design algorithm (used for ON-TARGETplus, Accell, siGENOME and Lincode siRNA) provides a high degree of confidence that the targeted gene will be the only one silenced.
The primary source of siRNA-mediated off-targets is the seed region (nucleotides 2-7), which uses the microRNA pathway to induce non-specific gene silencing via interactions within the 3' UTR. Incorporating comprehensive bioinformatic strategies such as seed region filters and seed frequency analysis within the SMARTselection design strategy minimizes microRNA-like off-target activity for improved specificity.
Pooling siRNA improves effectiveness
The SMARTpool (available for ON-TARGETplus, Accell, siGENOME and Lincode siRNA) combines four gene-specific siRNAs into a single reagent pool. Research has shown that pooled siRNAs:
- Improve the likelihood of effective gene silencing
- Minimize sequence-specific off-targeting by lowering the relative concentration of each siRNA
- Better mimic the natural RNAi pathway
- Reduce false negatives caused by inaccessible binding sites
Which siRNA is right for you?
Horizon offers multiple predesigned product lines across human, mouse and rat genomes. Use the table below to determine the optimal siRNA for your needs.
| ON-TARGETplus siRNA Chemically modified for enhanced specificity | Accell siRNA Self delivering siRNA | siGENOME siRNA Cost-effective, efficient silencing | Lincode siRNA Knockdown long non-coding RNA | |
|---|---|---|---|---|
| Recommended for neuronal, suspension, primary and other difficult-to-transfect cells | ||||
| Recommended transfection reagent | DharmaFECT transfection reagent | None required | DharmaFECT transfection reagent | DharmaFECT transfection reagent |
| Available as individual siRNA and SMARTpool formats | ||||
| Guaranteed knockdown by SMARTpool and 3 of 4 siRNAs | ||||
| Sense strand modifications to prevent interaction with RISC and favor antisense strand uptake | ||||
| Antisense strand seed region modifications to destabilize off-target activity and enhance target specificity | ||||
| Modified to facilitate cellular uptake without separate transfection reagents | ||||
| Stabilizing modifications to prevent nuclease-mediated degradation | ||||
| Sequence information provided with purchase | ||||
| Search ON-TARGETplus | Search Accell | Search siGENOME | Search Lincode |
Scale up your siRNA experiment
Run your own siRNA knockdown screen at any scale with our predesigned and custom library options.
Human siRNA libraries
Find predesigned ON-TARGETplus and Accell libraries for druggable, gene family and whole genome screening.
Mouse siRNA libraries
Find predesigned ON-TARGETplus and siGENOME libraries for druggable, gene family and whole genome screening.
Cherry-Pick custom library builder
Design your own siRNA screening library and only screen the genes you need.
Experimental support
Need help with a new RNAi technique? We offer live scientific support as well as an extensive collection of technical resources to help get you started!
Speak with an RNAi expert
Our scientific support team of PhD scientists is here to help with your gene knockdown experiment.
siRNA technical resources
Posters, app notes, protocols and technical manuals for using siRNA solutions by Dharmacon to achieve reliable and efficient gene silencing.
Video: Basic siRNA resuspension
Learn the best practice for resuspending lyophilized siRNA in this short video.
Dharmacon siRNA selection guide
Choose the optimal siRNA product line and format for your experiment
Accell siRNA silencing workflow
Experiment workflow for gene silencing with Accell self-delivering siRNA.
siRNA control recommendations
This technical note discusses the function and features of a variety of control siRNAs.
*Gene silencing guarantee applies to SMARTpool and “3 of 4 individual siRNA” formats when demonstrated to have been used under optimal delivery conditions (confirmed using validated positive control and measured at the mRNA level 24 to 48 hours after transfection using 100nM siRNA). For more information regarding the siRNA knockdown guarantee, please contact tech support.