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RNA interference via synthetic small interfering RNA (siRNA) is the preferred choice for fast but reliable loss-of-function studies. Broadly useful across many applications and in any cell line, siRNA offers a straightforward and widely cited technique for functional gene silencing. For over 20 years, Dharmacon has remained the most trusted name in high quality siRNA design and synthesis.
Select a predesigned siRNA product line or design your own siRNA with custom modifications.
The ON-TARGETplus, Accell and siGENOME siRNA lines are guaranteed* to effectively silence target gene expression by at least 75% at the mRNA level.
Knockdown long, non-coding RNA genes with specialized siRNA, modified to ensure specificity.
Design your own siRNA for any species on our user-friendly tool.
A successful siRNA knockdown experiment must be both effective and specific. Horizon’s SMARTselection design algorithm (used for ON-TARGETplus, Accell, siGENOME and Lincode siRNA) provides a high degree of confidence that the targeted gene will be the only one silenced.
The primary source of siRNA-mediated off-targets is the seed region (nucleotides 2-7), which uses the microRNA pathway to induce non-specific gene silencing via interactions within the 3' UTR. Incorporating comprehensive bioinformatic strategies such as seed region filters and seed frequency analysis within the SMARTselection design strategy minimizes microRNA-like off-target activity for improved specificity.
The SMARTpool (available for ON-TARGETplus, Accell, siGENOME and Lincode siRNA) combines four gene-specific siRNAs into a single reagent pool. Research has shown that pooled siRNAs:
Horizon offers multiple predesigned product lines across human, mouse and rat genomes. Use the table below to determine the optimal siRNA for your needs.
Chemically modified for enhanced specificity
Self delivering siRNA
Cost-effective, efficient silencing
Knockdown long non-coding RNA
|Recommended for neuronal, suspension, primary and other difficult-to-transfect cells|
|Recommended transfection reagent||DharmaFECT transfection reagent||None required||DharmaFECT transfection reagent||DharmaFECT transfection reagent
|Available as individual siRNA and SMARTpool formats|
|Guaranteed knockdown by SMARTpool and 3 of 4 siRNAs|
|Sense strand modifications to prevent interaction with RISC and favor antisense strand uptake|
|Antisense strand seed region modifications to destabilize off-target activity and enhance target specificity|
|Modified to facilitate cellular uptake without separate transfection reagents|
|Stabilizing modifications to prevent nuclease-mediated degradation|
|Sequence information provided with purchase|
|Search ON-TARGETplus||Search Accell||Search siGENOME||Search Lincode|
RNAi based screening using synthetic siRNA is a useful tool for understanding a disease pathway or for drug target identification. Researchers can design a custom screening library with as few as 20 ON-TARGETplus, Accell, Lincode or custom siRNA using our Cherry-Pick Library Tool, or browse our wide selection of predefined gene family and whole genome siRNA libraries.
Need help with a new RNAi technique? We offer live scientific technical support as well as an extensive collection of technical resources to help get you started!
Choose the optimal siRNA product line and format for your experiment