Trusted gene silencing from the longest standing siRNA guarantee on the market
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Expert design provides highly potent siRNA for all human, mouse, and rat genes.
- Guaranteed knockdown by SMARTpool and 3 of 4 individual siRNAs (see formats tab.)
- Rational strand bias approach to promote effective silencing and reduce sense strand off-targeting (see below).
- Sequence information provided with siRNA purchase.
Design and modification strategies for optimal silencing
In addition to identifying potent siRNA designs with the SMARTselection algorithm, the creation of siGENOME siRNAs also involves:
- Thermodynamic analysis to determine optimal antisense strand-loading characteristics for effective silencing.
- BLAST analysis of both strands to alleviate cross-targeting.
- To retain more high-scoring siRNA designs, the above analysis may trigger the selective use of a sense strand-blocking modification (ON-TARGET) to ensure only the appropriate strand enters RISC for effective target knockdown (see Supporting Data tab).
A robust selection of validated positive, negative, and novel RISC-Free® controls to effectively assess all aspects of RNAi experiments. Select a species-specific positive control to silence an expressed housekeeping gene in your experimental cell type. Our panel of non-targeting controls permits assessment of potential non-specific effects, to find the optimal negative control for your cell type, and your assay.
siGENOME Positive Control Reagents
A validated positive silencing control targeting the Cyclophilin B (PPIB) gene in human, mouse, or rat cell lines. Useful for determination of optimal RNAi experimental conditions.
A validated positive silencing control targeting the GAPD gene in human cell lines. Useful for determination of optimal RNAi experimental conditions.
A validated positive silencing control targeting the Lamin A/C genes in human, mouse, and rat cell lines. Useful for determination of optimal RNAi experimental conditions.
siGENOME Negative Control Reagents
Negative control siRNAs designed to target no known genes in human, mouse or rat. Recommended for determination of baseline cellular responses in RNAi experiments.
Negative control siRNA pools, each comprised of four siRNAs designed to target no known genes in human, mouse, or rat. Recommended for determination of baseline cellular responses in RNAi experiments.
An effective RNAi transfection control that will not be taken up by RISC. Provides a reliable baseline for cellular response to lipid-RNA complexes.
siGENOME Control siRNA Kits
Seven cross-species, validated siRNA controls provided at 5 nmol quantity. Includes positive and negative RNAi controls, labeled transfection indicator, and a negatives control for assessing baseline transfection effects
Eleven cross-species, validated siRNA controls provided at 5 nmol quantity. Includes positive and negative RNAi controls, labeled transfection indicator, and a negative control for assessing baseline transfection effects.
siGENOME siRNA Libraries
Find the predefined gene family, including Druggable genes or Whole Genome, that’s right for your discovery efforts.
A wide selection of predefined siRNA libraries, including the most up-to-date Whole Mouse genome collection available.
Human and mouse SMARTpool siRNA libraries in a novel pre-dispensed format to enable RNAi screening without the need for high-throughput automation.
Fast and easy online configuration and ordering of plated siRNA & microRNA reagents targeting your genes of interest.
No pre-designed product to fit your needs? Use our online design tools and extensive synthesis options to create a custom siRNA specific for your application.
- A mixture of 4 siRNA provided as a single reagent; providing advantages in both potency and specificity. siGENOME and ON-TARGETplus are guaranteed to silence by 75% or better.
Set of 4:
- A convenient option for purchasing aliquots of all 4 individual siRNAs targeting a single gene. Three of four siGENOME and ON-TARGETplus siRNAs are guaranteed† to silence by 75% or better.
- Select 1, 2, 3 or 4 individual siRNAs per gene.
- Predefined and Cherry-pick
- Non-targeting and Positive
Our siRNA knockdown guarantee
siGENOME siRNA reagents (SMARTpool and three of four individual siRNAs) are guaranteed to silence target gene expression by at least 75% at the mRNA level when demonstrated to have been used under optimal delivery conditions (confirmed using validated positive control and measured at the mRNA level 24 to 48 hours after transfection using 100nM siRNA).
|Approximate # reactions (wells) at 25 nM siRNA concentration (assuming no loss from pipetting)|
|nmol||96-well plate (100 uL total reaction volume)||24-well plate (500 uL total reaction volume)||12-well plate (1000 uL total reaction volume)|
Note: Most siGENOME and ON-TARGETplus siRNA products are highly functional at 5 to 25nM working concentration.
Which siRNA is right for you?
Dharmacon offers four complete pre-designed product lines across human, mouse and rat genomes. Use the table below to assist you in determining the right siRNA product line for your needs.
|Cost-effective, efficient silencing||High specificity for reduced off-targets + efficient silencing||Highly specific knockdown of long noncoding RNA (lncRNA)||Target silencing in difficult-to-transfect cells|
|siGENOME siRNA||ON-TARGETplus siRNA||Lincode siRNA||Accell siRNA|
|Pre-designed for Human, Mouse and Rat protein-coding genes||+||+||+|
|Pre-designed for Human and Mouse long noncoding RNA (lncRNA)||+|
|Recommended for transfectable mammalian cells in culture||+||+||+|
|Recommended for neuronal, suspension, primary and other difficult-to-transfect cells||+|
|Recommended transfection reagent||DharmaFECT transfection reagent||DharmaFECT transfection reagent||DharmaFECT transfection reagent||None required|
|Available as SMARTpool reagent||+||+||+||+|
|Available as four individual siRNAs||+||+||+||+|
|Guaranteed knockdown by SMARTpool and 3 of 4 siRNAs||+||+|
|Sense strand is modified to prevent interaction with RISC and favor antisense strand uptake||Selective application when thermodynamic analysis indicates it necessary for favorable antisense RISC loading||+||+||+|
|Antisense strand seed region is modified to destabilize off-target activity and enhance target specificity||+||+|
|Modifications to facilitate cellular uptake without separate transfection reagents||+|
|Stabilizing modifications to prevent nuclease-mediated degradation||+|
|Sequence information provided with purchase||+||+||+||+|
siGENOME SMARTpool reagents demonstrate potency at 5 nM working concentration
A comparison of target gene silencing at 5 and 100 nM concentration illustrates the high potency of siGENOME SMARTpool reagents. 5 or 100 nM siGENOME SMARTpool siRNA reagents targeting the indicated genes was transfected into HeLa cells (10K cells/well) using 0.1 ?L/well DharmaFECT 1. The negative control (NTC pool) was siGENOME Non-Targeting Pool #2.
Unnecessary sense-strand inactivation can increase off-target activity
Unmodified and sense strand-inactivated siRNAs were used to target five genes. The unmodified siRNAs have natural guide-strand loading characteristics. In four cases off-targets were increased due to enhanced RISC loading of the antisense strand when the sense strand was modified. All siRNAs had comparable silencing potency.Why not modify ALL siGENOME siRNAs to ensure proper strand loading? It has been demonstrated that forcing antisense (guide) strand entry into RISC may actually INCREASE off-targets due to increased loading of the guide strand and resulting off-target activity by its seed region. siGENOME siRNAs are designed with thermodynamic properties to naturally facilitate guide strand entry to RISC, which has been demonstrated to correlate with functionality. However, in cases where a high-scoring siGENOME siRNA does not possess ideal strand-loading characteristics, a sense (passenger) strand-inhibiting chemical modification (ON-TARGET) is utilized to promote guide strand entry.
Data shown represents genes down-regulated by twofold or more. HEK293 cells were transfected with 100 nM siRNA using 0.2 ?L of DharmaFECT 1. Data was analyzed at 24 hours by genomewide microarray analysis (Agilent).