Mimix™ KRAS G12S, 50% 1 ug Reference Standard

The KRAS G12S Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of assays that detect somatic mutations, such as Sanger and qPCR sequencing assays.

DISCONTINUATION NOTE: HD288 will be discontinued by 17-Apr-2025. Last order request date is on/before 07-Apr-2025. We are working to include this mutation in an upcoming KRAS multiplex panel which will be available from May/June 2025. In the meantime, you can use the following alternate products HD210/HD730. For any further queries please contact us. If you face any issues while placing the order, please reach out to orders@horizondiscovery.com or your local sales team or distributor.

Horizon DNA standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. The standard is provided at an allelic frequency of 50%, and may be diluted using the corresponding wild-type standard to generate a lower allelic frequencies for limit of detection studies. Horizon’s Base-Seq products are all derived from human cell lines, and are high molecular weight DNA. This format ensures that they may be applied to a wide-range of assays including qPCR, Sanger sequencing, next-generation sequencing, mass array, and more.

With this product you are able to:

  • Analyze the sensitivity of your assay
  • Gain certainty of the limit of detection
  • Optimize and validate new cancer panels and routinely monitor the performance of your assay

 

Technical Data

Synonyms: C-Ki-Ras, K-RAS2B, KI-RAS, KRAS2, RASK2

Format: Genomic DNA

DNA Base Change: GGT→AGT

NCBI Reference Assembly SNP: rs121913530

Buffer: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0

 

Product Information

Unit Size: 1µg

Concentration: 50ng/µl

 

General Information

Allelic Frequency: 50%

Storage: 4˚C

Expiry: See all product shelf life information

 

Quality Control

Allelic Frequency: Droplet Digital PCR™

Genotype: Sanger sequencing of locus specific PCR

Quality: Agarose gel electrophoresis

Quantification: Spectrophotometry (A260)

Genotype: KRAS (G12S/+)

Intended use: For assay developers and molecular diagnostic labs for routine performance monitoring of molecular biology assays. Research use only. Not for diagnostic procedures.