Horizon DNA standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. The standard is provided at an allelic frequency of 50%, and may be diluted using the corresponding wild-type standard to generate a lower allelic frequencies for limit of detection studies. Horizon’s Base-Seq products are all derived from human cell lines, and are high molecular weight DNA. This format ensures that they may be applied to a wide-range of assays including qPCR, Sanger sequencing, next-generation sequencing, mass array, and more.

With this product you are able to:

• Analyze the sensitivity of your assay
• Gain certainty of the limit of detection
• Optimize and validate new cancer panels and routinely monitor the performance of your assay

Technical Data

Synonyms: C-Ki-Ras, K-RAS2B, KI-RAS, KRAS2, RASK2

Format: Genomic DNA

DNA Base Change: GGT→AGT

NCBI Reference Assembly SNP: rs121913530

Cosmic ID: COSM517

Buffer: Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.50

Product Information

Intended Use: For routine performance monitoring (Research Use Only)

Unit Size: 1µg

Concentration: 50ng/µl

General Information

Allelic Frequency: 50%

Storage: 4˚C

Expiry: See all product shelf life information

Cell Line Background: SW48

Exome sequencing data: Download additional information on variants present in the parental cell lines

Quality Control

Allelic Frequency: Droplet Digital PCR™

Genotype: Sanger sequencing of locus specific PCR

Quality: Agarose gel electrophoresis

Quantification: Spectrophotometry (A260)

Genotype: KRAS (G12S/+)

Characterization

Gel Image:

DNA was run on a 1% agarose gel and shows a single high-molecular weight band

Chromatogram:

Chromatograph showing heterozygosity for the K-Ras G12S mutation within K-Ras exon 2 (SNP accession number: rs121913530)

Primers: