Prostate Cancer Panel FFPE Reference Standard
The Prostate Cancer Panel FFPE Reference Standard is a well-characterized, cell line-derived control material manufactured under ISO:13485 that contains 16 variants across 9 genes clinically relevant to prostate cancer. This innovative, multigene multiplex product allows labs to perform fast, reliable, and cost-effective validation of prostate cancer assays using FFPE samples, saving the user time and money to achieve their quality assurance goals faster. Additionally, it allows the user to assess the performance of FFPE-based assays crucial for routine clinical diagnostics. Like all Revvity Mimix reference standards, it closely mimics patient samples used in diagnostic workflows.
Batch-specific supplementary NGS data
The Prostate Cancer Panel FFPE Reference Standard comes with a batch specific supplementary NGS data. You can access the VCF file here. Each batch was sequenced using Agilent's CREV4 (Clinical Research Exome) kit at a depth of 250x on Illumina platform and run through the Illumina DRAGEN Somatic Pipeline for mapping, sorting, and variant calling.
Technical Data
Format: FFPE
Fixation Method: 4% PFA (w/v)
Section Size: 15µm
Cell Density: 3 x 108 cells per block. Approx. 3.5 x 105 cells per section
Expected DNA Yield: 400 ng using Promega Maxwell RSC FFPE Plus DNA Extraction kit
Genes covered: AR, BRCA1, BRCA2, CDKN2A, PIK3CA, PTEN, RB1, STK11, TP53
Allelic Frequencies: 2.50- 11.00%
Verified Mutations:
| Gene | DNA sequence change |
Amino acid change | Expected allelic frequency (%) |
|---|---|---|---|
| AR | c.2623C>T | p.H875Y | 2.50 |
| AR | c.2632A>G | p.T878A | 2.50 |
| BRCA1 | c.5329dup | p.Q1777Pfs*74 | 6.50 |
| BRCA2 | c.9924C>G | p.Y3308* | 6.00 |
| CDKN2A | c.250G>T | p.D84Y | 7.00 |
| PIK3CA | c.1637A>G | p.Q546R | 6.00 |
| PIK3CA | c.1624G>A | p.E542K | 8.00 |
| PTEN | c.17_18del | p.K6Rfs*4 | 4.00 |
| RB1 | c.2143A>T | p.K715* | 7.00 |
| STK11 | c.532_536del | p.K178fs*86 | 10.00 |
| TP53 | c.992A>G | p.Q331R | 7.00 |
| TP53 | c.742C>T | p.R248W | 11.00 |
| TP53 | c.668C>T | p.P223L | 4.00 |
| TP53 | c.820G>T | p.V274F | 8.00 |
| TP53 | c.414del | p.K139Rfs*31 | 8.00 |
| AR | CNV-Gain | N/A | 5.00 copies |
General information
Storage: 4°C
Shipping: Ambient
Expiration: See all product shelf life information
Intended use: For assay developers, molecular diagnostic labs (Research use only)
Quality control
Allelic frequency: Droplet Digital™ PCR
Quantification: Quantifluor
DNA Integrity: Agarose gel electrophoresis
Manufacturing Quality: ISO 9001:2015 and ISO 13485:2016
We accept orders using the following methods.
Online
Our online catalog and ordering system can be used to search our full menu of available Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.
Offline
If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order. We recommend that you use our online system to identify the products you require and their respective “HD” product codes.
To order offline we will require the following information:
- Product code(s) of the item(s) you wish to order (e.g. "HD123")
- Your name, email address, title and organization
- Shipping address and billing address with contact names
- Purchase Order number if you have one
- Telephone number
- VAT Number (European Countries only)
If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please contact us.
Additional Resources
Certificate of analysis
Safety data sheets
Selection guides
Reference Standards
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At what temperature do you ship reference standards?
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How can I open the NGS data .gz files?
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How do you manufacture your Horizon Reference Standards?
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How do you recommend extracting DNA from an FFPE reference standard?
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How was the batch-specific supplementary NGS data produced and analysed?
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In which formats is the Prostate Cancer Panel DNA Reference Standard available, and do they all contain the PTEN loss variant?
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Is it possible to order custom versions of Horizon Reference Standard?
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Is there a wildtype control in the same background and format as the Prostate Cancer Panel FFPE Reference Standard (HD848)?
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The FFPE yield is lower than expected, how can I improve the yield?
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What batch-specific supplementary NGS data is provided?
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What is the difference between the variants on the CoA and those in the supplementary batch-specific NGS data?
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Where can I access batch-specific supplementary NGS data?
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Which Droplet Digital PCR (ddPCR) system is used for variant detection at Horizon?
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Why do I see different allele frequencies or different variants in my NGS data when comparing to the data provided with the reference standard?