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- Mimix™ Prostate Cancer Panel FFPE Reference Standard
Mimix™ Prostate Cancer Panel FFPE Reference Standard
The Prostate Cancer Panel FFPE Reference Standard is a well-characterized, cell line-derived control material manufactured under ISO:13485 that contains 16 variants across 9 genes clinically relevant to prostate cancer. This innovative, multigene multiplex product allows labs to perform fast, reliable, and cost-effective validation of prostate cancer assays using FFPE samples, saving the user time and money to achieve their quality assurance goals faster. Additionally, it allows the user to assess the performance of FFPE-based assays crucial for routine clinical diagnostics. Like all Revvity Mimix reference standards, it closely mimics patient samples used in diagnostic workflows.
Batch-specific supplementary NGS data
The Prostate Cancer Panel FFPE Reference Standard comes with a batch specific supplementary NGS data. You can access the VCF file here. Each batch was sequenced using Agilent's CREV4 (Clinical Research Exome) kit at a depth of 250x on Illumina platform and run through the Illumina DRAGEN Somatic Pipeline for mapping, sorting, and variant calling.
Technical Data
Format: FFPE
Fixation Method: 4% PFA (w/v)
Section Size: 15µm
Cell Density: 3 x 108 cells per block. Approx. 3.5 x 105 cells per section
Expected DNA Yield: 400 ng using Promega Maxwell RSC FFPE Plus DNA Extraction kit
Genes covered: AR, BRCA1, BRCA2, CDKN2A, PIK3CA, PTEN, RB1, STK11, TP53
Allelic Frequencies: 2.50- 11.00%
Verified Mutations:
Gene | DNA sequence change |
Amino acid change | Expected allelic frequency (%) |
---|---|---|---|
AR | c.2623C>T | p.H875Y | 2.50 |
AR | c.2632A>G | p.T878A | 2.50 |
BRCA1 | c.5329dup | p.Q1777Pfs*74 | 6.50 |
BRCA2 | c.9924C>G | p.Y3308* | 6.00 |
CDKN2A | c.250G>T | p.D84Y | 7.00 |
PIK3CA | c.1637A>G | p.Q546R | 6.00 |
PIK3CA | c.1624G>A | p.E542K | 8.00 |
PTEN | c.17_18del | p.K6Rfs*4 | 4.00 |
RB1 | c.2143A>T | p.K715* | 7.00 |
STK11 | c.532_536del | p.K178fs*86 | 10.00 |
TP53 | c.992A>G | p.Q331R | 7.00 |
TP53 | c.742C>T | p.R248W | 11.00 |
TP53 | c.668C>T | p.P223L | 4.00 |
TP53 | c.820G>T | p.V274F | 8.00 |
TP53 | c.414del | p.K139Rfs*31 | 8.00 |
AR | CNV-Gain | N/A | 5.00 copies |
General information
Storage: 4°C
Shipping: Ambient
Expiration: See all product shelf life information
Intended use: For assay developers, molecular diagnostic labs (Research use only)
Quality control
Allelic frequency: Droplet Digital™ PCR
Quantification: Quantifluor
DNA Integrity: Agarose gel electrophoresis
Manufacturing Quality: ISO 9001:2015 and ISO 13485:2016
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